PTH1R/PTHR1 Antibody (3D1.1)

Western Blot: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type and Ramp2-/- placentas. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY licence.

Immunohistochemistry: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Immunofluorescence for PTH1R in the labyrinth of wild type and Ramp2-/- placentas. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Analysis of anti-PTH1R antibody with human liver at concentration 5 ug/ml.

Immunohistochemistry: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Immunofluorescence for PTH1R in the labyrinth of wild type and Ramp2-/- placentas. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Po, Bv, Ca, Eq, Pm, Pm, Rt, Bt, Ch, Ha, Ma-Op, Pm-Or, Rb
• Applications: WB, ICC/IF, IHC, IHC-P
• Clone: 3D1.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

PTH1R/PTHR1 Antibody (3D1.1) Summary

• Immunogen: Synthetic peptide corresponding to aa146-169 of human PTH1R/PTHR1 [RRCDRNGSWELVPGHNRTWANYSE]. Species sequence Homology: Mouse & rat (23/24aa).
• Epitope: aa146-169 [RRCDRNGSWELVPGHNRTWANYSE]
• Specificity: Recognizes human PTH/PTHrP receptor, MW 80kD, and the receptor dimer, Mr 160kD. A faint nonspecific band at 110kD is also detected. This antibody does not detect the PTH2 receptor.
• Predicted Species: Rat (96%), Hamster (96%), Chicken (92%), Bat (96%), Opossum (92%), Orangutan (100%), Rabbit (96%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTH1R
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5-2 ug/ml
• Application Notes: Use in ICC/IF reported in scientific literature (PMID 28727763).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28727763). Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Xenopus (88%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for PTH1R/PTHR1 Antibody (3D1.1)

• MGC138426
• parathyroid hormone 1 receptorMGC138452
• parathyroid hormone/parathyroid hormone-related peptide receptor
• parathyroid hormone/parathyroid hormone-related protein receptor
• PFE
• PTH/PTHr receptor
• PTH/PTHrP type I receptor
• PTH1 receptor
• PTH1R
• PTHR
• PTHR1
• PTHRPTHR1parathyroid hormone receptor 1
• seven transmembrane helix receptor

Background

PTHR1, a Parathyroid Hormone Receptor, mediates PTH-dependent regulation of mineral-ion homeostasis. It also mediates the paracrine action of PTHRP in endochondral bone formation. Mutations in this receptor are responsible for disorders of calcium and bone metabolism such as chondrodysplasia, hypercalcemia, and chronic renal failure. Three promoters, P1, P2, and P3, regulate the expression of PTHR. PTHR1 has been reported to be expressed in bone, breast, brain, colon, GI, kidney, liver, placenta, skin, umbilical cord, and uterus. ESTs have been isolated from B-cell/lung/testis, brain, breast, fetus, heart, heart/melanocyte/uterus, kidney, liver/spleen, lung, placenta, and testis libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTH1R/PTHR1 Antibody (NBP1-40067)(3)

Publications using NBP1-40067

• Dole, N S, Yee, C S Et al. TGF beta Regulation of Perilacunar/Canalicular Remodeling Is Sexually Dimorphic. J Bone Miner Res Aug 1 2020 [PMID: 32282961] (Flow, Human)
• Kadmiel M, Matson BC, Espenschied ST et al. Loss of receptor activity-modifying protein 2 in mice causes placental dysfunction and alters PTH1R regulation PLoS ONE 2017 Jul 20 [PMID: 28727763] (ICC/IF, Mouse)
• Madiraju P, Gawri R, Wang H et al. Mechanism of parathyroid hormone-mediated suppression of calcification markers in human intervertebral disc cells. Eur Cell Mater 2013 May 2 [PMID: 23636951] (WB, Human)

Bioinformatics

• Gene Symbol: PTH1R
• Entrez:
  • 5745(Human)
• Uniprot:
  • Q03431()