NGFR/TNFRSF16/p75NTR Antibody [Unconjugated]

Recombinant Human/Mouse/Rat NGF R/TNFRSF16 (Catalog # 367-NR) was serially diluted 2-fold and captured by Goat Anti-Human/Mouse/Rat NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF367) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse NGF R/TNFRSF16 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF367) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

NGFR/TNFRSF16/p75NTR Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NGF R/TNFRSF16
  Lys29-Asn250
  Accession # P08138
• Specificity: Detects human NGF R/TNFRSF16 in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse NGF R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NGFR
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NGFR/TNFRSF16/p75NTR Antibody [Unconjugated]

• CD271 antigen
• CD271
• Gp80-LNGFR
• member 16
• nerve growth factor receptor
• NGF R
• NGF receptor
• NGFR
• p75 ICD
• p75 NTR
• TNFRSF16
• TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16)
• tumor necrosis factor receptor superfamily member 16

Background

p75 neurotrophin receptor, also named low affinity NGF receptor (NGF R), is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3, and NT-4 approximately equally with low affinity. The cytoplasmic region contains a subtype 2 death domain.

NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma and in amniotic fluid of humans and rats.

1. Barker, P.A. and R.A. Murphy (1992) Molecular and Cellular Biochemistry 110:1.
2. Bamji, A.X. et al. (1998) J. Cell Biol. 140:911.
3. Feinstein, E. et al. (1995) Trends Biochem. Sci. 20:342.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NGFR/TNFRSF16/p75NTR Antibody (AF367)(5)

Publications using AF367

• JP Zanin, JL Verpeut, Y Li, MW Shiflett, SS Wang, V Santhakuma, WJ Friedman The p75NTR influences cerebellar circuit development and adult behavior via regulation of cell cycle duration of granule cell progenitors J. Neurosci., 2019-10-03;0(0):. 10/3/2019 [PMID: 31582529]
• Kathryn Ellis, Elizabeth C. Driver, Takayuki Okano, Abigail Lemons, Matthew W. Kelley GSK3 regulates hair cell fate in the developing mammalian cochlea Developmental Biology 9/1/2019 [PMID: 31185200]
• Kathryn Ellis, Elizabeth C. Driver, Takayuki Okano, Abigail Lemons, Matthew W. Kelley GSK3 regulates hair cell fate in the developing mammalian cochlea Developmental Biology 2019-09-01 [PMID: 31185200] (Immunohistochemistry, Transgenic Mouse)
• Agustin Anastasia, Phillip A Barker, Moses V Chao, Barbara L Hempstead Detection of p75NTR Trimers: Implications for Receptor Stoichiometry and Activation Journal of Neuroscience 8/26/2015 [PMID: 26311773]
• Ramnaraine M, Pan W, Goblirsch M, Lynch C, Lewis V, Orchard P, Mantyh P, Clohisy DR Direct and bystander killing of sarcomas by novel cytosine deaminase fusion gene. Cancer Res., 2003-10-15;63(20):6847-54. 2003-10-15 [PMID: 14583482] (Western Blot, Human)

Bioinformatics

• Gene Symbol: NGFR
• Uniprot:
  • P08138()