NGFR/TNFRSF16/p75NTR Antibody (MLR2)

Product Discontinued

NGFR/TNFRSF16/p75NTR Antibody (MLR2) Summary

• Immunogen: Extracellular domain of human p75NTR
• Specificity: Specificity has been confirmed using a number of techniques as described in the reference by Rogers et al (2006).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NGFR
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 20 ug/ml
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1-5 ug/ml
• Application Notes: This antibody is useful in Immunohistochemistry-Frozen, Immunofluorescence and Flow Cytometry,and Immunopanning. A concentration of 1-5 ug/ml is recommended for immunohistochemistry, immunopanning. A concentration of 20 ug/ml is recommended for immunofluorescence and Flow Cytometry. The optimal working dilution should be determined by the end user.

Reactivity Notes

This antibody is known to react with human, mouse, rat and guinea-pig p75NTR protein.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: No buffer
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with deionized water. Glycerol (1:1) may be added.

Alternate Names for NGFR/TNFRSF16/p75NTR Antibody (MLR2)

• CD271 antigen
• CD271
• Gp80-LNGFR
• member 16
• nerve growth factor receptor
• NGF R
• NGF receptor
• NGFR
• p75 ICD
• p75 NTR
• TNFRSF16
• TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16)
• tumor necrosis factor receptor superfamily member 16

Background

p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR is not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. DOMAIN: Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. PTM: Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. SIMILARITY: Contains 4 TNFR-Cys repeats. This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NGFR/TNFRSF16/p75NTR Antibody (M-009-100)(2)

Publications using M-009-100

• 1Wiese S, Herrmann T, Drepper C, Jablonka S, Funk N, Klausmeyer A, Rogers ML, Rush RA & Sendtner M. (2009) Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos. Nat Protoc;5(1):31-8. 2010-01-01 [PMID: 20057379]
• Rogers ML, Atmosukarto I, Berhanu DA, Matusica D, Macardle P, Rush RA. Functional monoclonal antibodies to p75 neurotrophin receptor raised in knockout mice. J Neurosci Methods;158(1):109-20. 2006-11-15 [PMID: 16828166]

Bioinformatics

• Gene Symbol: NGFR
• Entrez:
  • 4804(Human)
  • 18053(Mouse)
  • 24596(Rat)
• Uniprot:
  • P08138()