NFkB p100/p52 Antibody

Western Blot: NFkB p100/p52 Antibody [NB100-82063] - Western blot analysis NF- kB p100/p52 (Ab-871) using NB100-82063 in HT-29 cells treated with TNF- a (20ng/ml, 15min) and Calyculin A (50nM, 15min).

Immunocytochemistry/Immunofluorescence: NFkB p100/p52 Antibody [NB100-82063] - Staining of methanol-fixed Hela cells using NFkB-p100 (Ab-872) antibody.

Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NB100-82063] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NFkB-p100/p52 (Ab-871) antibody.

Western Blot: NFkB p100/p52 Antibody [NB100-82063] - Extracts from HT29 cells using NFkB-p100(Ab-872) Antibody and the same antibody preincubated with blocking peptide

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

NFkB p100/p52 Antibody Summary

• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human NFkB p100/p52 around amino acids 870~874 (S-Q-S-V-E).
• Specificity: Detects endogenous levels of total NFkB p100/p52 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFKB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100~1:200
  • Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NFkB p105/p50 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NFkB p100/p52 Antibody

• DNA-binding factor KBF2
• H2TF1
• Lymphocyte translocation chromosome 10 protein
• Lyt10
• LYT10LYT-10
• nuclear factor NF-kappa-B p100 subunit
• nuclear factor of kappa light chain gene enhancer in B-cells 2
• nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2
• Oncogene Lyt-10
• p52

Background

NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NFkB p100/p52 Antibody (NB100-82063)(1)

Publication using NB100-82063

• Connolly NP, Galisteo R, Xu S et al. Elevated fibroblast growth factor-inducible 14 expression transforms proneural-like gliomas into more aggressive and lethal brain cancer Glia 2021-05-15 [PMID: 33991013]

Bioinformatics

• Gene Symbol: NFKB2
• Entrez:
  • 4791(Human)
• Uniprot:
  • Q00653()