NFkB p100/p52 Antibody

Images

 
Western Blot: NFkB p100/p52 Antibody [NB100-82063] - Extracts from HT29 cells using NFkB-p100(Ab-872) Antibody and the same antibody preincubated with blocking peptide
Immunocytochemistry/ Immunofluorescence: NFkB p100/p52 Antibody [NB100-82063] - Staining of methanol-fixed Hela cells using NFkB-p100 (Ab-872) antibody.
Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NB100-82063] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NFkB-p100/p52 (Ab-871) antibody.
Western Blot: NFkB p100/p52 Antibody [NB100-82063] - Western blot analysis NF- kB p100/p52 (Ab-871) using NB100-82063 in HT-29 cells treated with TNF- a (20ng/ml, 15min) and Calyculin A (50nM, 15min).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

NFkB p100/p52 Antibody Summary

Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human NFkB- p100/p52 around aa. 872 (S-Q-S-V-E).
Specificity
NFkB-p100/p52 (specific to a.a. 871) detects endogenous levels of total NFkB-p100/p52 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFKB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100~1:200
  • Immunohistochemistry 1:50~1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NFkB p105/p50 Lysate (NBL1-13619)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NFkB p100/p52 Antibody

  • DNA-binding factor KBF2
  • H2TF1
  • Lymphocyte translocation chromosome 10 protein
  • Lyt10
  • LYT10LYT-10
  • nuclear factor NF-kappa-B p100 subunit
  • nuclear factor of kappa light chain gene enhancer in B-cells 2
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2
  • Oncogene Lyt-10
  • p52

Background

NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NFkB p100/p52 Antibody (NB100-82063) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on NFkB p100/p52.

Nuclear Factor kappa B Signaling in the Immune System
Nuclear Factor kappa B (NFkB) binds to the kappa-beta site of the immunoglobulin kappa light chain gene enhancer. Thus NFkB has become one of the widely studied transcription factors in innate and adaptive immune responses. The NFkB family is composed...  Read full blog post.

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Bioinformatics

Gene Symbol NFKB2
Entrez
Uniprot