NFIL3 Antibody

Western Blot: NFIL3 Antibody [NB100-842] - Analysis of NFIL3 in HepG2 lysate using NB100-842 at 0.5ug/ml (RIPA buffer, 20ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

Flow Cytometry: NFIL3 Antibody [NB100-842] - analysis of paraformaldehyde fixed Human peripheral blood mononuclear cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NFIL3 Antibody Summary

• Description: Novus Biologicals Goat NFIL3 Antibody (NB100-842) is a polyclonal antibody validated for use in IHC, WB, ELISA and Flow. Anti-NFIL3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RLIATQPISASDSG corresponding to C-Terminus according to NP_005375.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NFIL3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1:1000
• Application Notes: Western blot: Approx. 60kDa band observed in nuclear lysates of cell line Jurkat and HeLa (calculated MW of 51.5kDa according to NP_005375.2). The observed molecular weight corresponds to different antibodies from other commercial sources. An additional band of 30kDa was observed in lysates of cell line HeLa, which was completely blocked with the immunizing peptide. Primary incubation 1 hour at room temperature
  Flow Cytometry: Flow cytometric analysis of Human peripheral blood mononuclear cells

Reactivity Notes

Expected from sequence similarity: Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NFIL3 Antibody

• E4BP4
• E4BP4IL3BP1
• IL3BP1
• Interleukin-3 promoter transcriptional activator
• Interleukin-3-binding protein 1
• NFIL3
• NF-IL3A
• NFIL3AE4 promoter-binding protein 4
• nuclear factor interleukin 3 regulated protein
• nuclear factor interleukin-3-regulated protein
• nuclear factor, interleukin 3 regulated
• Transcriptional activator NF-IL3A

Background

A basic leucine zipper transcription factor, which activates IL3 gene expression and can also repress transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NFIL3 Antibody (NB100-842)(1)

Publication using NB100-842

• Zhang W, Zhang J, Kornuc M et al. Molecular cloning and characterization of NF-IL3A, a transcriptional activator of the human interleukin-3 promoter. Mol Cell Biol 1995-11-01 [PMID: 7565758]

Bioinformatics

• Gene Symbol: NFIL3
• Entrez:
  • 4783(Human)
  • 18030(Mouse)
  • 114519(Rat)
• Uniprot:
  • NP_005375.(Human)