NF-M Antibody - BSA Free

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Analysis in human cerebral cortex and liver tissues. Corresponding NF-M RNA-seq data are presented for the same tissues.

Western Blot: NF-M Antibody [NBP1-87754] - Analysis NBP1-87754 (A) shows similar pattern to independent antibody NBP1-87753 (B).

Immunocytochemistry/Immunofluorescence: NF-M Antibody [NBP1-87754] - Staining of human cell line HEK 293 shows localization to intermediate filaments. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human liver shows no positivity in hepatocytes as expected.

Western Blot: NF-M Antibody [NBP1-87754] - Analysis in human cell line HEK 293 and human cell line A-431.

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human cerebellum shows strong cytoplasmic positivity in white matter.

Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NF-M Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KEEEEKEVKEAPKEEKVEKKEEKPKDVPEKKKAESPVKEEAVAEVVTITKSVKVHLEKETKEEGKPLQQEKEKEKAGGEGGSEEEGSDKGAKG
• Marker: Neuronal Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEFM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
• Control Peptide:

  NF-M Protein (NBP1-87754PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NF-M Antibody - BSA Free

• NEF3
• NEF3neurofilament medium polypeptide
• NEFM
• Neurofilament 3
• neurofilament, medium polypeptide 150kDa
• neurofilament, medium polypeptide
• neurofilament-3 (150 kD medium)
• NFM
• NF-M
• NF-M160 kDa neurofilament protein
• NFMNeurofilament triplet M protein

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some size variation in across species.

Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NF-M Antibody (NBP1-87754)(2)

Publications using NBP1-87754

• Haggmark A, Mikus M, Mohsenchian A et al. Plasma profiling reveals three proteins associated to amyotrophic lateral sclerosis. Ann Clin Transl Neurol 2014-08-01 [PMID: 25356426]
• Bachmann J, Burte F, Pramana S et al. Affinity Proteomics Reveals Elevated Muscle Proteins in Plasma of Children with Cerebral Malaria. PLoS Pathog 2014-04-01 [PMID: 24743550]

Bioinformatics

• Gene Symbol: NEFM