Genetic Strategies: Knockout Validated: NF-M Antibody [NB300-133] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NF-M knockout (KO) HeLa cell line. PVDF membrane ...read more
Immunocytochemistry/ Immunofluorescence: NF-M Antibody [NB300-133] - Analysis of rat cerebellum section stained with rabbit pAb to NF-M, NB300-133, dilution 1:2,000 in red, and costained with mouse mAb to GAP43, ...read more
Western Blot: NF-M Antibody [NB300-133] - Analysis of neuronal tissue lysates using rabbit pAb to NF-M, NB300-133, dilution 1:2,000 in green:  protein standard (red),  rat brain,  rat spinal cord,  mouse ...read more
Immunohistochemistry-Frozen: NF-M Antibody [NB300-133] - IHC-Fr staining of cerebral cortex section from Rat with NF-M antibody (red) at 1:5000 dilution. This immunostaining reveals the perikarya of pyramidal neurons ...read more
Western Blot: NF-M Antibody [NB300-133] - 160kDa Neurofilament Medium Antibody [NB300-133] - Western blot analysis of 160kDa Neurofilament Medium expression on whole rat cerebellum homogenate using NB300-133.
Immunocytochemistry/ Immunofluorescence: NF-M Antibody [NB300-133] - Mixed neuron/glia cultures stained with NB300-133 (red) and NBP2-25141 (green), Novus' mouse monoclonal antibody to Fox1, an mRNA binding protein ...read more
This 160kDa Neurofilament Medium Antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, and Immunohistochemistry-Frozen. In Western blot a band can be seen around 145kDa corresponding to rodent Neurofilament Medium. Human and bovine Neurofilament Medium run a little slower and can be seen at about 160kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
160 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
5mM Sodium Azide
Alternate Names for NF-M Antibody
NEF3neurofilament medium polypeptide
neurofilament, medium polypeptide 150kDa
neurofilament, medium polypeptide
neurofilament-3 (150 kD medium)
NF-M160 kDa neurofilament protein
NFMNeurofilament triplet M protein
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called Neurofilament Light (NF-L), Neurofilament Medium (NF-M) and Neurofilament Heavy (NF-H). Neurofilament Medium is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some variation in different species. Antibodies to Neurofilament Medium are useful to identify this protein and identify neurons and their processes in tissue sections and in tissue culture. Neurofilament Medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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