NF-M Antibody

Images

 
Western Blot: 160kDa Neurofilament Medium Antibody [NB300-133] - Western blot analysis of 160kDa Neurofilament Medium expression on whole rat cerebellum homogenate using NB300-133.
Immunocytochemistry/ Immunofluorescence: NF-M Antibody [NB300-133] - Mixed neuron/glia cultures stained with NB300-133 (red) and NBP2-25141 (green), Novus' mouse monoclonal antibody to Fox1, an mRNA binding protein ...read more
Immunohistochemistry-Frozen: NF-M Antibody [NB300-133] - IHC-Fr staining of cerebral cortex section from Rat with NF-M antibody (red) at 1:5000 dilution. This immunostaining reveals the perikarya of pyramidal neurons ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Av, Bv, FeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NF-M Antibody Summary

Immunogen
Recombinant rat Neurofilament Medium fusion protein corresponding to the C-terminus [UniProt# P12839]
Marker
Neuronal Marker
Specificity
Specifically recognizes the evolutionarily conserved extreme C-terminal region of Neurofilament Medium (~145-170kDa).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NEFM
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Frozen 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
Application Notes
This 160kDa Neurofilament Medium Antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, and Immunohistochemistry-Frozen. In Western blot a band can be seen around 145kDa corresponding to rodent Neurofilament Medium. Human and bovine Neurofilament Medium run a little slower and can be seen at about 160kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NF-M Lysate (NBL1-13578)
Publications
Read Publications using
NB300-133 in the following applications:

Reactivity Notes

Human, mouse, rat, avian, bovine, feline and porcine. Predicted to react with all mammals.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for NF-M Antibody

  • NEF3
  • NEF3neurofilament medium polypeptide
  • NEFM
  • Neurofilament 3
  • neurofilament, medium polypeptide 150kDa
  • neurofilament, medium polypeptide
  • neurofilament-3 (150 kD medium)
  • NFM
  • NF-M
  • NF-M160 kDa neurofilament protein
  • NFMNeurofilament triplet M protein

Background

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called Neurofilament Light (NF-L), Neurofilament Medium (NF-M) and Neurofilament Heavy (NF-H). Neurofilament Medium is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some variation in different species. Antibodies to Neurofilament Medium are useful to identify this protein and identify neurons and their processes in tissue sections and in tissue culture. Neurofilament Medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NF-M Antibody (NB300-133)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(3)
IHC
(1)
IHC-P
(1)
WB
(1)
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Human
(1)
Mouse
(3)
Rat
(3)
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Showing Publications 1 - 7 of 7.
Publications using NB300-133 Applications Species
Sikora J, Dworski S, Jones EE et al. Acid Ceramidase Deficiency in Mice Results in a Broad Range of Central Nervous System Abnormalities. Am. J. Pathol. Apr 1 2017 [PMID: 28342444] (ICC/IF, Mouse) ICC/IF Mouse
Li LS, Liu CZ, Xu JD et al. Effect of entacapone on colon motility and ion transport in a rat model of Parkinson's disease. World J Gastroenterol 2015 Mar 28 [PMID: 25834315] (Rat) Rat
From R, Eilam R, Bar-Lev DD et al. Oligodendrogenesis and myelinogenesis during postnatal development effect of glatiramer acetate. Glia 2014 Feb 13 [PMID: 24481644] (IHC-P, Mouse) IHC-P Mouse
Liu HX, Ermilov A, Grachtchouk M et al. Multiple Shh signaling centers participate in fungiform papilla and taste bud formation and maintenance. Dev Biol 2013 Aug 2 [PMID: 23916850] (IHC, Mouse) IHC Mouse
Elan D Louis, Karen, Rachel Babij, Etty Cortes, Ronald K Liem, Jean-Paul G Vonsattel, Phyllis L Faust. Neurofilament Protein Levels: Quantitative Analysis in Essential Tremor Cerebellar Cortex,. Neuroscience Letters, Available online, 10.1016/j.neulet.2012.04.054. 2012 may4. [PMID: 22561033] (WB, Human) WB Human
Ziv-Polat O, Skaat H, Shahar A, Margel S. Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering Int J Nanomedicine 2012 [PMID: 22419873] (ICC/IF, Rat) ICC/IF Rat
Skaat H, Ziv-Polat O, Shahar A, Margel S. Enhancement of the growth and differentiation of nasal olfactory mucosa cells by the conjugation of growth factors to functional nanoparticles. Bioconjug Chem;22(12):2600-10. 2011 Dec 21. [PMID: 22029397] (ICC/IF, Rat) ICC/IF Rat

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NEFM
Entrez
Uniprot