Western Blot: 160kDa Neurofilament Medium Antibody [NB300-133] - Western blot analysis of 160kDa Neurofilament Medium expression on whole rat cerebellum homogenate using NB300-133.
Immunocytochemistry/ Immunofluorescence: NF-M Antibody [NB300-133] - Mixed neuron/glia cultures stained with NB300-133 (red) and NBP2-25141 (green), Novus' mouse monoclonal antibody to Fox1, an mRNA binding protein ...read more
Immunohistochemistry-Frozen: NF-M Antibody [NB300-133] - IHC-Fr staining of cerebral cortex section from Rat with NF-M antibody (red) at 1:5000 dilution. This immunostaining reveals the perikarya of pyramidal neurons ...read more
This 160kDa Neurofilament Medium Antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, and Immunohistochemistry-Frozen. In Western blot a band can be seen around 145kDa corresponding to rodent Neurofilament Medium. Human and bovine Neurofilament Medium run a little slower and can be seen at about 160kDa.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
160 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human, mouse, rat, avian, bovine, feline and porcine. Predicted to react with all mammals.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Alternate Names for NF-M Antibody
NEF3neurofilament medium polypeptide
neurofilament, medium polypeptide 150kDa
neurofilament, medium polypeptide
neurofilament-3 (150 kD medium)
NF-M160 kDa neurofilament protein
NFMNeurofilament triplet M protein
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called Neurofilament Light (NF-L), Neurofilament Medium (NF-M) and Neurofilament Heavy (NF-H). Neurofilament Medium is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some variation in different species. Antibodies to Neurofilament Medium are useful to identify this protein and identify neurons and their processes in tissue sections and in tissue culture. Neurofilament Medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our NF-M Antibody and receive a gift card or discount.