NF-H Antibody (321721)

• Reactivity: Hu
• Applications: WB
• Clone: 321721
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NF-H Antibody (321721) Summary

• Immunogen: E. coli-derived recombinant human NF-H
  Met1-Ala380
  Accession # P12036
• Specificity: Detects human NF-H in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) NF-L or rhNF-M is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEFH
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NF-H Antibody (321721)

• 200 kDa neurofilament protein
• KIAA0845
• NEFH
• Neurofilament Heavy (200kDa)
• neurofilament heavy polypeptide
• Neurofilament triplet H protein
• neurofilament, heavy polypeptide 200kDa
• neurofilament, heavy polypeptide
• NF200
• NFH
• NF-H

Background

The human Neurofilament Heavy chain, also known as NF-H and NEFH, is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. In the region used for immunization, mouse and rat NF-H are each 93% identical to human NF-H.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEFH
• Uniprot:
  • P12036()