Neuropilin-1 Antibody (M534)

Western Blot: Neuropilin-1 Antibody (M534) [NBP3-23440] - Western blot image of recombinant human Neuropilin-1 (lanes 1 & 3) and human PC3 cells (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-Neuropilin-1 (NBP3-23182) (lanes 1 & 2) or with mouse monoclonal anti-Neuropilin-1 (NBP3-23440) (lanes 3 & 4).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: M534
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Neuropilin-1 Antibody (M534) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone M534 was generated from a neuropilin-1 synthetic peptide (coupled to carrier protein) corresponding to amino acids within the a1 CUB domain of human neuropilin-1. This sequence is highly conserved in rat and mouse neuropilin-1, and has low homology to neuropilin-2. [UniProt# O14786]
• Specificity: The antibody detects a 130 kDa protein corresponding to the apparent molecular mass of neuropilin-1 on SDS-PAGE immunoblots of human recombinant neuropilin-1 and in human PC3 cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NRP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Western Blot 1:250
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Neuropilin-1 Antibody (M534)

• BDCA4
• BDCA-4
• CD304 antigen
• CD304
• DKFZp686A03134
• DKFZp781F1414
• neuropilin 1
• Neuropilin1
• Neuropilin-1
• NP1
• NRP
• NRP1
• transmembrane receptor
• Vascular endothelial cell growth factor 165 receptor
• VEGF165R

Background

Neuropilin-1/CD304 is a type-I transmembrane protein. It forms a heterodimeric complex with vascular endothelial growth factor receptor 2, and this complex functions as a receptor for vascular endothelial growth factor. On the other hand, Neuropilin-1/CD304 forms a complex with plexin-1, and this complex acts as a semaphorin 3A receptor. Neuropilin-1/CD304 is involved in angiogenesis, axon guidance, cell survival, migration, and invasion. Recently, it has been reported that Neuropilin-1/CD304 is an essential component located on the immunological synapse and acts as a potent cell-surface marker for murine regulatory T cells (Treg). Neuropilin-1/CD304 expressed by Treg cells promotes long interactions between Treg cells and immature dendritic cells and restricts access of effector cells to the antigen-presenting cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Neuropilin-1 Antibody (NBP3-23440). (Showing 1 - 2 of 2 FAQ).

1. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
   • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
2. What research areas can Neuropilin-1 antibodies be used in?
   • Neuropilin-1 products can be applied in the following research areas: Adaptive Immunity, Angiogenesis, Breast Cancer, Cancer, Cardiovascular Biology, Neuroscience, and Virology Bacteria and Parasites.

Bioinformatics

• Gene Symbol: NRP1