Neurofascin Antibody

Western Blot: Neurofascin Antibody [NBP3-13182] - Various tissue extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Neurofascin antibody (NBP3-13182) diluted at 1:2000.

Immunohistochemistry-Frozen: Neurofascin Antibody [NBP3-13182] - Neurofascin antibody detects Neurofascin protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: Neurofascin protein stained by Neurofascin antibody (NBP3-13182) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: Neurofascin Antibody [NBP3-13182] - Neurofascin antibody detects Neurofascin protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Neurofascin antibody (NBP3-13182) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Neurofascin Antibody [NBP3-13182] - Neurofascin antibody detects Neurofascin protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Neurofascin antibody (NBP3-13182) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Neurofascin antibody detects Neurofascin protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Neurofascin protein stained by Neurofascin antibody (NBP3-13182) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Mu, Rt, Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Neurofascin Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Neurofascin. The exact sequence is proprietary.
• Predicted Species: Human (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFASC
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 138 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Neurofascin Antibody

• DKFZp686P2250
• KIAA0756FLJ46866
• neurofascin homolog (chicken)
• neurofascin homolog
• Neurofascin
• NF
• NFASC
• NRCAML

Background

NFASC (Neurofascin) is involved in cell adhesion, axon subcellular targeting, synapse formation, regulating functions of the brain, and axonal guidance. NFASC is known to have interactions with SDCBP, ABL1, ANK1, CRK and DCX. NFASC has been studied in relation to multiple sclerosis, carcinoma, neuronitis and Parkinson's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFASC