| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3235-SP | |||
HAF007). Specific bands were detected for Neurofascin at approximately 140 to 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of rat brain tissue, mouse brain tissue, human brain (cortex) tissue, human brain (cerebellum) tissue, and human brain (hippocampus) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Neurofascin Monoclonal Antibody (Catalog # MAB3235) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Neurofascin at approximately 140 to 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3235-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81886-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95191-0.02ml | |||
![P0T124M mutation alters SLI morphology, length,&distribution. (D) Quantification shows an increased %age of SLIs in nerves from mice harboring the P0T124M mutation (MpzT124M) at 2, 6, 12,&18 months of age. One-way ANOVA [2 month old: F (2, 10) = 29.86, p<0.0001; 6 month old: F (2, 9) = 41.60, p<0.0001, 12 month old: F (2, 6) = 34.35, p = 0.0005; 18 month old: F (2, 8) = 21.71, p = 0.0006]. Representative confocal pictures of sciatic teased fibers stained with FITC-phalloidin (ACTIN, green) (E, I,&M) or anti-MAG antibodies (Q) (green)&anti-pan-NFASC antibodies (red) at 2 (E), 6 (I),&12 (M&Q) months of age. SLI morphology is disrupted in MpzT124M mice. Scale bar: 10 μm. Measurements of SLI length at 2 (F), 6 (J),&12 (N&R) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 32.16, p <0.0001; 6 month old: F (2,6) = 11.18, p = 0.0095 12 month old: F (2,9) = 6.447, p = 0.0183]. Measurements of the distance between adjacent SLI at 2 (G), 6 (K),&12 (O&S) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 24.90, p = 0.0002; 6 month old: F (2,6) = 9.382, p = 0.0142; 12 month old: F (2,9) = 13.04, p = 0.0022]. Quantifications of SLI number per 100 μm at 2 (H), 6 (L),&12 (P&T) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 20.29, p = 0.0005; 6 month old: F (2,6) = 22.81, p = 0.016; 12 month old: F (2,9) = 28.25, p = 0.0001]. At least 207 SLI per genotype quantified at each time point. n (animals) ≥ 3 per genotype. *p < 0.05, **p < 0.01, ***p < 0.001 by multiple-comparisons Tukey’s post hoc tests after Nested one-way ANOVA (D, F, G, H, J, K, L, R, S,&T) or by two-tailed Student’s t test (N, O,&P). Graphs indicate means ± SEMs. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36350884), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af3235_human-mouse-rat-neurofascin-affinity-purified-polyclonal-ab-8120255541769.jpg)
![P0T124M mutation alters nodes and paranodes.Representative confocal pictures of sciatic nerve teased fibers stained with antibodies against the paranodal marker CASPR (red) and the paranodal and nodal marker pan-Neurofascin (NFASC; green) at 2 (A) and 12 (E) months of age. Scale bars: 5 μm. CASPR length quantifications at 2 (B) and 12 (F) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 7.009, p = 0.0146; 12 month old: F (2,7) = 35.47, p = 0.0002]. Relative frequency distributions of paranodal (CASPR) length at 2 (C) and 12 (G) months of age. Nodal length quantifications at 2 (D) and 12 (H) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 1.122, p = 0.3671, 12 month old: F (2,7) = 8.012, p = 0.0155]. At least 200 paranodes and 100 nodes per genotype were quantified at each time point. n (animals) ≥ 3 per genotype. (I) Electron micrographs of ultrathin longitudinal WT and MpzT124M/T124M sciatic nerve section at 12 months of age. In (I) electron micrographs represent nodes of WT and MpzT124M/T124M sciatic nerves. (J) The quantification of nodal length shows significant widening of the node in MpzT124M/T124M. Magnifications of (I) show disorganized paranonal loops in MpzT124M/T124M but well organized in WT. n (animals) ≥ 3 per genotype, 7 to 11 nodes were counted per animals, for a total of 27 and 28 counted by genotype. Scale bare: top panel 1 μm, middle panel 500 nm, bottom panel 200 nm. ***p < 0.001 by multiple-comparisons Tukey’s post hoc tests after Nested one-way ANOVA (B, F, D, and H) or Nested two-tailed Student’s t test (J). Graphs indicate means ± SEMs. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36350884), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af3235_human-mouse-rat-neurofascin-affinity-purified-polyclonal-ab-8120255552818.jpg)
![Western Blot: Neurofascin Antibody [NBP3-13182] - Various tissue extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Neurofascin antibody (NBP3-13182) diluted at 1:2000.](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Western-Blot-NBP3-13182-img0001.jpg "Western Blot: Neurofascin Antibody [NBP3-13182] - Various tissue extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Neurofascin antibody (NBP3-13182) diluted at 1:2000.")
![Immunohistochemistry-Frozen: Neurofascin Antibody [NBP3-13182] - Neurofascin antibody detects Neurofascin protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: Neurofascin protein stained by Neurofascin antibody (NBP3-13182) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Immunohistochemistry-Frozen-NBP3-13182-img0005.jpg)
![Immunohistochemistry-Paraffin: Neurofascin Antibody [NBP3-13182] - Neurofascin antibody detects Neurofascin protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Neurofascin antibody (NBP3-13182) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Immunohistochemistry-Paraffin-NBP3-13182-img0002.jpg)
![Immunohistochemistry-Paraffin: Neurofascin Antibody [NBP3-13182] - Neurofascin antibody detects Neurofascin protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Neurofascin antibody (NBP3-13182) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Immunohistochemistry-Paraffin-NBP3-13182-img0003.jpg)
![Neurofascin antibody detects Neurofascin protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Neurofascin protein stained by Neurofascin antibody (NBP3-13182) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13182_rabbit-neurofascin-pab-immunocytochemistry-immunofluorescence-261120251822432.jpg)
![Western Blot: Neurofascin Antibody [NBP1-78794] - (0.5ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Western-Blot-NBP1-78794-img0003.jpg "Western Blot: Neurofascin Antibody [NBP1-78794] - (0.5ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Neurofascin Antibody [NBP2-95191] - Analysis of extracts of various cell lines, using Neurofascin at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Western-Blot-NBP2-95191-img0001.jpg "Western Blot: Neurofascin Antibody [NBP2-95191] - Analysis of extracts of various cell lines, using Neurofascin at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Western Blot: Neurofascin Antibody (S07-0B3) [NBP3-19519] - Western blot detection of Neurofascin in Mouse Brain, Rat Brain, CSO7, Hela cell lysates using NBP3-19519 (1:1000 diluted). Predicted band size: 150kDa. Observed band size: 186, 155, 75kDa.](http://images.novusbio.com/fullsize/Neurofascin-Antibody-S07-0B3-Western-Blot-NBP3-19519-img0001.jpg "Western Blot: Neurofascin Antibody (S07-0B3) [NBP3-19519] - Western blot detection of Neurofascin in Mouse Brain, Rat Brain, CSO7, Hela cell lysates using NBP3-19519 (1:1000 diluted). Predicted band size: 150kDa. Observed band size: 186, 155, 75kDa.")
![ELISA: Human Neurofascin ELISA Kit (Colorimetric) [NBP3-39128] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39128_human-neurofascin-elisa-kit-colorimetric-2110202415334913.png "ELISA: Human Neurofascin ELISA Kit (Colorimetric) [NBP3-39128] - Standard Curve Reference")
![ELISA: Human Neurofascin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39129] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39129_human-neurofascin-ready-to-use-elisa-kit-colorimetric-2110202415302912.png "ELISA: Human Neurofascin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39129] - Standard Curve Reference")
