| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3235-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3235-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81886-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95191-0.02ml | |||
![P0T124M mutation alters SLI morphology, length,&distribution. (D) Quantification shows an increased %age of SLIs in nerves from mice harboring the P0T124M mutation (MpzT124M) at 2, 6, 12,&18 months of age. One-way ANOVA [2 month old: F (2, 10) = 29.86, p<0.0001; 6 month old: F (2, 9) = 41.60, p<0.0001, 12 month old: F (2, 6) = 34.35, p = 0.0005; 18 month old: F (2, 8) = 21.71, p = 0.0006]. Representative confocal pictures of sciatic teased fibers stained with FITC-phalloidin (ACTIN, green) (E, I,&M) or anti-MAG antibodies (Q) (green)&anti-pan-NFASC antibodies (red) at 2 (E), 6 (I),&12 (M&Q) months of age. SLI morphology is disrupted in MpzT124M mice. Scale bar: 10 μm. Measurements of SLI length at 2 (F), 6 (J),&12 (N&R) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 32.16, p <0.0001; 6 month old: F (2,6) = 11.18, p = 0.0095 12 month old: F (2,9) = 6.447, p = 0.0183]. Measurements of the distance between adjacent SLI at 2 (G), 6 (K),&12 (O&S) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 24.90, p = 0.0002; 6 month old: F (2,6) = 9.382, p = 0.0142; 12 month old: F (2,9) = 13.04, p = 0.0022]. Quantifications of SLI number per 100 μm at 2 (H), 6 (L),&12 (P&T) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 20.29, p = 0.0005; 6 month old: F (2,6) = 22.81, p = 0.016; 12 month old: F (2,9) = 28.25, p = 0.0001]. At least 207 SLI per genotype quantified at each time point. n (animals) ≥ 3 per genotype. *p < 0.05, **p < 0.01, ***p < 0.001 by multiple-comparisons Tukey’s post hoc tests after Nested one-way ANOVA (D, F, G, H, J, K, L, R, S,&T) or by two-tailed Student’s t test (N, O,&P). Graphs indicate means ± SEMs. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36350884), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af3235_human-mouse-rat-neurofascin-affinity-purified-polyclonal-ab-8120255541769.jpg)
![P0T124M mutation alters nodes and paranodes.Representative confocal pictures of sciatic nerve teased fibers stained with antibodies against the paranodal marker CASPR (red) and the paranodal and nodal marker pan-Neurofascin (NFASC; green) at 2 (A) and 12 (E) months of age. Scale bars: 5 μm. CASPR length quantifications at 2 (B) and 12 (F) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 7.009, p = 0.0146; 12 month old: F (2,7) = 35.47, p = 0.0002]. Relative frequency distributions of paranodal (CASPR) length at 2 (C) and 12 (G) months of age. Nodal length quantifications at 2 (D) and 12 (H) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 1.122, p = 0.3671, 12 month old: F (2,7) = 8.012, p = 0.0155]. At least 200 paranodes and 100 nodes per genotype were quantified at each time point. n (animals) ≥ 3 per genotype. (I) Electron micrographs of ultrathin longitudinal WT and MpzT124M/T124M sciatic nerve section at 12 months of age. In (I) electron micrographs represent nodes of WT and MpzT124M/T124M sciatic nerves. (J) The quantification of nodal length shows significant widening of the node in MpzT124M/T124M. Magnifications of (I) show disorganized paranonal loops in MpzT124M/T124M but well organized in WT. n (animals) ≥ 3 per genotype, 7 to 11 nodes were counted per animals, for a total of 27 and 28 counted by genotype. Scale bare: top panel 1 μm, middle panel 500 nm, bottom panel 200 nm. ***p < 0.001 by multiple-comparisons Tukey’s post hoc tests after Nested one-way ANOVA (B, F, D, and H) or Nested two-tailed Student’s t test (J). Graphs indicate means ± SEMs. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36350884), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af3235_human-mouse-rat-neurofascin-affinity-purified-polyclonal-ab-8120255552818.jpg)
![Western Blot: Neurofascin Antibody [NBP3-13182] - Various tissue extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Neurofascin antibody (NBP3-13182) diluted at 1:2000.](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Western-Blot-NBP3-13182-img0001.jpg "Western Blot: Neurofascin Antibody [NBP3-13182] - Various tissue extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Neurofascin antibody (NBP3-13182) diluted at 1:2000.")
![Immunohistochemistry-Frozen: Neurofascin Antibody [NBP3-13182] - Neurofascin antibody detects Neurofascin protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: Neurofascin protein stained by Neurofascin antibody (NBP3-13182) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Immunohistochemistry-Frozen-NBP3-13182-img0005.jpg)
![Immunohistochemistry-Paraffin: Neurofascin Antibody [NBP3-13182] - Neurofascin antibody detects Neurofascin protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Neurofascin antibody (NBP3-13182) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Immunohistochemistry-Paraffin-NBP3-13182-img0002.jpg)
![Immunohistochemistry-Paraffin: Neurofascin Antibody [NBP3-13182] - Neurofascin antibody detects Neurofascin protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Neurofascin antibody (NBP3-13182) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Immunohistochemistry-Paraffin-NBP3-13182-img0003.jpg)
![Neurofascin antibody detects Neurofascin protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Neurofascin protein stained by Neurofascin antibody (NBP3-13182) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13182_rabbit-neurofascin-pab-immunocytochemistry-immunofluorescence-261120251822432.jpg)
![Immunohistochemistry-Paraffin: Neurofascin Antibody [NBP1-81886] - Staining in human cerebral cortex and pancreas tissues using anti-NFASC antibody. Corresponding NFASC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Immunohistochemistry-Paraffin-NBP1-81886-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurofascin Antibody [NBP1-81886] - Staining in human cerebral cortex and pancreas tissues using anti-NFASC antibody. Corresponding NFASC RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurofascin Antibody [NBP1-81886] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Immunohistochemistry-Paraffin-NBP1-81886-img0007.jpg)
![Immunohistochemistry-Paraffin: Neurofascin Antibody [NBP1-81886] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Immunohistochemistry-Paraffin-NBP1-81886-img0006.jpg)
![Western Blot: Neurofascin Antibody [NBP1-78794] - (0.5ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Western-Blot-NBP1-78794-img0003.jpg "Western Blot: Neurofascin Antibody [NBP1-78794] - (0.5ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Neurofascin Antibody [NBP2-95191] - Analysis of extracts of various cell lines, using Neurofascin at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/Neurofascin-Antibody-Western-Blot-NBP2-95191-img0001.jpg "Western Blot: Neurofascin Antibody [NBP2-95191] - Analysis of extracts of various cell lines, using Neurofascin at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Western Blot: Neurofascin Antibody (S07-0B3) [NBP3-19519] - Western blot detection of Neurofascin in Mouse Brain, Rat Brain, CSO7, Hela cell lysates using NBP3-19519 (1:1000 diluted). Predicted band size: 150kDa. Observed band size: 186, 155, 75kDa.](http://images.novusbio.com/fullsize/Neurofascin-Antibody-S07-0B3-Western-Blot-NBP3-19519-img0001.jpg "Western Blot: Neurofascin Antibody (S07-0B3) [NBP3-19519] - Western blot detection of Neurofascin in Mouse Brain, Rat Brain, CSO7, Hela cell lysates using NBP3-19519 (1:1000 diluted). Predicted band size: 150kDa. Observed band size: 186, 155, 75kDa.")
![ELISA: Human Neurofascin ELISA Kit (Colorimetric) [NBP3-39128] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39128_human-neurofascin-elisa-kit-colorimetric-2110202415334913.png "ELISA: Human Neurofascin ELISA Kit (Colorimetric) [NBP3-39128] - Standard Curve Reference")
![ELISA: Human Neurofascin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39129] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39129_human-neurofascin-ready-to-use-elisa-kit-colorimetric-2110202415302912.png "ELISA: Human Neurofascin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39129] - Standard Curve Reference")