Netrin-G1a Antibody (1010035) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with Netrin-G1a (filled histogram) or irrelevant protein (open histogram) was stained with Mouse Anti-Human Netrin-G1a Monoclonal Antibody (Catalog # MAB10019) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

U87-MG human glioblastoma cell line was stained with Mouse Anti-Human Netrin-G1a Monoclonal Antibody (Catalog # MAB10019) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 1010035
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Netrin-G1a Antibody (1010035) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived human Netrin-G1a
  His29-Gly503
  Accession # Q9Y212
• Specificity: Detects human Netrin-G1a in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Netrin-G1a Antibody (1010035) [Unconjugated]

• Lmnt1
• NetrinG1a
• Netrin-G1a
• NTG1
• NTNG1

Background

NTNG-1, known as Netrin-G1 or alternatively Laminet-1 (Gene name: NTNG1), is a member of the UNC-6/Netrin family of proteins. Human Netrin-G1 is a 55 kDa protein and shares 100% homology with its crab-eating macaque, 98% homology with its rat homolog, and 97% homology with its mouse homolog. Netrin-G1 is synthesized as a 539 amino acid (aa) precursor with a 28 aa signal sequence, a 251 aa laminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, a GPI (glycosylphosphatidylinisotol) anchor (Ser510), and a C-terminal 28 aa propeptide that is removed in mature Netrin-G1 (1, 3, 4). Netrin-G1 interacts with its ligand, Netrin-G ligand 1 (NGL1) to regulate neuron growth and patterning, axonal subdendritic differentiation, and synapse formation throughout development (1, 2, 4, 6). Abnormal expression of Netrin-G1 via SNPs (single nucleotide polymorphisms) have been implicated in pathogenesis for schizophrenia (5). Netrin-G1 has widespread expression only in vertebrates, occurring in olfactory mitral cells, cells of the inferior colliculus (hearing), dorsal thalamus (behavior), and cells of the deep cerebellar nuclei and inferior olive (motion) (3).

1. Seiradake, E. et al. (2011) EMBO J. 30:4479.
2. Song, Y. et al. (2013) J Cell Sci. 126:4926.
3. Nakashiba, T. et al. (2000) J Neuroscience. 20:6540.
4. Matsukawa, H. et al. (2014) J Neuroscience. 34:15779.
5. Zhu, Y. et al. (2011) J Genet. 90:499.
6. Nishimura-Akiyoshi, S. et al. (2007) Neuroscience. 104:14801.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9Y212()