NGL-1/LRRC4C Antibody

Western Blot: NGL-1/LRRC4C Antibody [NBP2-19516] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NGL-1/LRRC4C Antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: NGL-1/LRRC4C Antibody [NBP2-19516] - Analysis of methanol-fixed MCF-7, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: NGL-1/LRRC4C Antibody [NBP2-19516] - Human breast cancer, using Netrin G1 ligand antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: NGL-1/LRRC4C Antibody [NBP2-19516] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:2000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NGL-1/LRRC4C Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Netrin G1 ligand. The exact sequence is proprietary.
• Localization: Membrane; Single-pass type I membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRRC4C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for NGL-1/LRRC4C Antibody

• KIAA1580NGL-1NGL1Netrin-G1 ligand
• leucine rich repeat containing 4C
• leucine-rich repeat-containing protein 4C
• LRRC4C
• NGL1
• NGL-1

Background

NGL1 is a specific binding partner for netrin G1 (NTNG1; MIM 608818), which is a member of the netrin family of axon guidance molecules (Lin et al., 2003 [PubMed 14595443]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: LRRC4C
• Uniprot:
  • Q9HCJ2(Human)