Netrin-1 Antibody [Unconjugated]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Netrin-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Netrin-1
Val22-Ala603
Accession # AAC52971
Specificity
Detects mouse Netrin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant chicken (rch) Netrin‑1 and recombinant human Netrin-1 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Netrin-G1a and rmNetrin-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NTN1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1109 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.



This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Netrin-1 Antibody [Unconjugated]

  • netrin 1
  • Netrin1
  • Netrin-1
  • NTN1
  • NTN1Lnetrin 1, mouse, homolog of

Background

Mouse Netrin-1 is a member of the laminin-related family of axon-guidance molecules, collectively referred to as Netrins (netr is Sanskrit for "one who guides"). The molecule's cDNA encodes a 603 amino acid (aa) protein precursor that has structural similarity to the N-terminal gamma -chain of laminin. It contains a globular domain, three EGF repeats, and a C-terminal heparin-binding domain. Mouse Netrin-1 shares 52% aa identity with mouse Netrin-3, and 98% and 87% aa identity with human and chicken Netrin-1, respectively. Cells reported to express Netrin-1 in the embryo include cells of the floor plate, ventricular zone of the spinal cord, the brain, the ganglionic eminence, and parts of the diencephalon. Netrins were first identified for promoting the outgrowth of commissural axons and are also involved in helping migrating cells and axonal growth cones navigate to their targets. Netrins can provide both attractive and repulsive cues to neurons, depending on the receptors present and cellular context. In the adult, Netrin-1 is likely involved in axon regeneration in peripheral nerves. Netrin-1 has also been shown to be expressed outside of the nervous system and to be involved in development of such tissues as the pancreas, lung, bowel, bone and mammary gland. In non-neural organogenesis, Netrin‑1 provides an adhesive rather than guidance function. The DCC (deleted in colorectal carcinoma), Neogenin, the UNC5 family of receptors, and the adenosine A2b receptors are proposed to be functional receptors for Netrin-1 (1-7).

  1. Puschel, A. (1999) Mech. Dev. 83:65.
  2. Hedgecock, E. and C. Norris (1997) Trends Genet. 13:251.
  3. Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
  4. Madison, R. et al. (2000) Exp. Neurology 161:563.
  5. Srinivasan, K. et al. (2003) Dev. Cell 4:371.
  6. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
  7. Corset, V. et al. (2000) Nature 407:747.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Netrin-1 Antibody (AF1109)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC-P, Neutralization, Western Blot.


Filter By Application
ICC
(1)
IHC-P
(1)
Neutralization
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(2)
Mouse
(5)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF1109 Applications Species
K Yamahara, T Nakagawa, J Ito, K Kinoshita, K Omori, N Yamamoto Netrin 1 mediates protective effects exerted by insulin-like growth factor 1 on cochlear hair cells Neuropharmacology, 2017;119(0):26-39. 2017 [PMID: 28373074] (Neutralization, Mouse) Neutralization Mouse
T Lahlali, ML Plissonnie, C Romero-Lóp, M Michelet, B Ducarouge, A Berzal-Her, F Zoulim, P Mehlen, R Parent Netrin-1 Protects Hepatocytes Against Cell Death Through Sustained Translation During the Unfolded Protein Response Cell Mol Gastroenterol Hepatol, 2016;2(3):281-301.e9. 2016 [PMID: 28174720] (Western Blot, Mouse) Western Blot Mouse
Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nat Med, 2014;20(4):377-84. 2014 [PMID: 24584118] (IHC-P, Mouse) IHC-P Mouse
van Gils , Janine M, Ramkhelawon , Bhama, Fernandes , Luciana, Stewart , Merran C, Guo , Liang, Seibert , Tara, Menezes , Gustavo, Cara , Denise C, Chow , Camille, Kinane , T Bernar, Fisher , Edward A, Balcells , Mercedes, Alvarez-Leite , Jacqueli, Lacy-Hulbert , Adam, Moore , Kathryn Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions. Arterioscler Thromb Vasc Biol, 2013;33(5):911-9. 2013 [PMID: 23430612] (Western Blot, Human) Western Blot Human
Shimizu A, Nakayama H, Wang P, Konig C, Akino T, Sandlund J, Coma S, Italiano J, Mammoto A, Bielenberg D, Klagsbrun M Netrin-1 promotes glioblastoma cell invasiveness and angiogenesis by multiple pathways including activation of RhoA, cathepsin B, and cAMP-response element-binding protein. J Biol Chem, 2013;288(4):2210-22. 2013 [PMID: 23195957] (Western Blot, Human) Western Blot Human
Mille F, Llambi F, Guix C, Delloye-Bourgeois C, Guenebeaud C, Castro-Obregon S, Bredesen DE, Thibert C, Mehlen P Interfering with multimerization of netrin-1 receptors triggers tumor cell death. Cell Death Differ., 2009;16(10):1344-51. 2009 [PMID: 19543238] (ICC, Mouse) ICC Mouse
Causeret F, Hidalgo-Sanchez M, Fort P, Backer S, Popoff MR, Gauthier-Rouviere C, Bloch-Gallego E Distinct roles of Rac1/Cdc42 and Rho/Rock for axon outgrowth and nucleokinesis of precerebellar neurons toward netrin 1. Development, 2004;131(12):2841-52. 2004 [PMID: 15151987] (Western Blot, Mouse) Western Blot Mouse

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF1109

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Bioinformatics

Gene Symbol NTN1
Uniprot