Netrin-1 Antibody (158936) [Unconjugated]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
158936
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

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Netrin-1 Antibody (158936) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Netrin-1
Val22-Ala603
Accession # AAC52971
Specificity
Detects mouse Netrin-1 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant chicken (rch) Netrin‑1 and no cross-reactivity with rchNetrin-2, recombinant human Netrin-4, recombinant mouse (rm) Netrin-4, or rmNetrin‑G1a is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
NTN1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB1109 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Netrin-1 Antibody (158936) [Unconjugated]

  • netrin 1
  • Netrin1
  • Netrin-1
  • NTN1
  • NTN1Lnetrin 1, mouse, homolog of

Background

Mouse Netrin-1 is a member of the laminin-related family of axon-guidance molecules, collectively referred to as Netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 603 amino acid (aa) protein precursor that has structural similarity to the N-terminal gamma -chain of laminin. It contains a globular domain, three EGF repeats, and a C-terminal heparin-binding domain. Mouse Netrin-1 shares 52% aa identity with mouse Netrin-3, and 98% and 87% aa identity with human and chicken Netrin-1, respectively. Cells reported to express Netrin-1 in the embryo include cells of the floor plate, ventricular zone of the spinal cord, the brain, the ganglionic eminence, and parts of the diencephalon. Netrins were first identified for promoting the outgrowth of commissural axons and are also involved in helping migrating cells and axonal growth cones navigate to their targets. Netrins can provide both attractive and repulsive cues to neurons, depending on the receptors present and cellular context. In the adult, Netrin-1 is likely involved in axon regeneration in peripheral nerves. Netrin‑1 has also been shown to be expressed outside of the nervous system and to be involved in development of such tissues as the pancreas, lung, bowel, bone and mammary gland. In non-neural organogenesis,
Netrin‑1 provides an adhesive rather than guidance function. The DCC (deleted in colorectal carcinoma), Neogenin, the UNC5 family of receptors, and the adenosine A2b receptors are proposed to be functional receptors for Netrin-1 (1-7).

  1. Puschel, A. (1999) Mech. Dev. 83:65.
  2. Hedgecock, E. and C. Norris (1997) Trends Genet. 13:251.
  3. Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
  4. Madison, R. et al. (2000) Exp. Neurology 161:563.
  5. Srinivasan, K. et al. (2003) Dev. Cell 4:371.
  6. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
  7. Corset, V. et al. (2000) Nature 407:747.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Netrin-1 Antibody (MAB1109)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-Fr, Western Blot.


Filter By Application
IHC-Fr
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(3)
Mouse
(4)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB1109 Applications Species
P Mehrpouya-, K Miranda, NP Singh, EE Zumbrun, M Nagarkatti, PS Nagarkatti Role of microRNA in CB1 antagonist-mediated regulation of adipose tissue macrophage polarization and chemotaxis during diet-induced obesity J. Biol. Chem., 2019;0(0):. 2019 [PMID: 30910812] (Western Blot, Mouse) Western Blot Mouse
A Méneret, EA Franz, O Trouillard, TC Oliver, Y Zagar, SP Robertson, Q Welniarz, RJM Gardner, C Gallea, M Srour, C Depienne, CL Jasoni, C Dubacq, F Riant, JC Lamy, MP Morel, R Guérois, J Andreani, C Fouquet, M Doulazmi, M Vidailhet, GA Rouleau, A Brice, A Chédotal, I Dusart, E Roze, D Markie Mutations in the netrin-1 gene cause congenital mirror movements J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28945198] (Western Blot, Human) Western Blot Human
C Dominici, JA Moreno-Bra, SR Puiggros, Q Rappeneau, N Rama, P Vieugue, A Bernet, P Mehlen, A Chédotal Floor-plate-derived netrin-1 is dispensable for commissural axon guidance Nature, 2017;0(0):. 2017 [PMID: 28445456] (Western Blot, Human) Western Blot Human
T Lahlali, ML Plissonnie, C Romero-Lóp, M Michelet, B Ducarouge, A Berzal-Her, F Zoulim, P Mehlen, R Parent Netrin-1 Protects Hepatocytes Against Cell Death Through Sustained Translation During the Unfolded Protein Response Cell Mol Gastroenterol Hepatol, 2016;2(3):281-301.e9. 2016 [PMID: 28174720] (IHC-Fr, Mouse) IHC-Fr Mouse
Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nat Med, 2014;20(4):377-84. 2014 [PMID: 24584118] (Western Blot, Mouse) Western Blot Mouse
Mancino M, Esposito C, Watanabe K, Nagaoka T, Gonzales M, Bianco C, Normanno N, Salomon DS, Strizzi L Neuronal guidance protein Netrin-1 induces differentiation in human embryonal carcinoma cells. Cancer Res., 2009;69(5):1717-21. 2009 [PMID: 19223540] (Western Blot, Human) Western Blot Human
Lourenco FC, Galvan V, Fombonne J, Corset V, Llambi F, Muller U, Bredesen DE, Mehlen P Netrin-1 interacts with amyloid precursor protein and regulates amyloid-beta production. Cell Death Differ., 2009;16(5):655-63. 2009 [PMID: 19148186] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol NTN1
Uniprot