Nesfatin-1/Nucleobindin-2 Antibody (877140) [Unconjugated]

HAF005). A specific band was detected for Nesfatin-1/ Nucleobindin-2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF005). A specific band was detected for Nesfatin-1/ Nucleobindin-2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of AGS human gastric adenocarcinoma cell line and human testis tissue. PVDF membrane was probed with 3 µg/mL of Rat Anti-Human Nesfatin-1/Nucleobindin-2 Monoclonal Antibody (Catalog # MAB5949) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Nesfatin-1/ Nucleobindin-2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of AGS human gastric adenocarcinoma cell line and human testis tissue. PVDF membrane was probed with 3 µg/mL of Rat Anti-Human Nesfatin-1/Nucleobindin-2 Monoclonal Antibody (Catalog # MAB5949) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to Golgi. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." > NL013) and counterstained with DAPI (blue). Specific staining was localized to Golgi. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." title="Nesfatin-1/Nucleobindin-2 was detected in immersion fixed human mesenchymal stem cells differentiated into adipocytes using Rat Anti-Human Nesfatin-1/Nucleobindin-2 Monoclonal Antibody (Catalog # MAB5949) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to Golgi. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." />

Nesfatin-1/Nucleobindin-2 was detected in immersion fixed human mesenchymal stem cells differentiated into adipocytes using Rat Anti-Human Nesfatin-1/Nucleobindin-2 Monoclonal Antibody (Catalog # MAB5949) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="Nesfatin-1/Nucleobindin-2 was detected in immersion fixed paraffin-embedded sections of human stomach using Rat Anti-Human Nesfatin-1/ Nucleobindin-2 Monoclonal Antibody (Catalog # MAB5949) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

Nesfatin-1/Nucleobindin-2 was detected in immersion fixed paraffin-embedded sections of human stomach using Rat Anti-Human Nesfatin-1/ Nucleobindin-2 Monoclonal Antibody (Catalog # MAB5949) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 877140
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Nesfatin-1/Nucleobindin-2 Antibody (877140) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Nesfatin-1/Nucleobindin-2
  Val25-Leu106
  Accession # P80303
• Specificity: Detects human Nesfatin-1/Nucleobindin-2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: NUCB2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 3 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nesfatin-1/Nucleobindin-2 Antibody (877140) [Unconjugated]

• DNA-binding protein NEFA
• NEFA
• NEFAGastric cancer antigen Zg4
• Nesfatin1
• Nesfatin-1
• NUCB2
• nucleobindin 2
• Nucleobindin-2
• nucleobinding 2

Background

Nesfatin-1 (NEFA Encoded Satiety and Fat-influencing protein 1) is a secreted, 10 kDa peptide derived from the translation product of the NUCB2 gene. Nesfatin-1 is associated with neurons involved in feeding (ARH and PVH) and fluid intake (SON and PVH), in beta cells of the pancreas, and in adipocytes. Its presence peripherally has an anorexigenic effect. Mature human Nesfatin-1 is 82 amino acids (aa) in length. It is the secreted N-terminus of a larger, intracellular 420 aa precursor termed NEFA/Nucleobindin-2/NUCB2. NUCB2 contains a signal sequence (aa 1‑24), Nesfatin-1 (aa 25‑106), a DNA-binding site (aa 171‑223), and two EF-hand regions (aa 241‑276 and 293‑328). Intracellular uncleaved NUCB2 is 45‑50 kDa in size. There is a potential for multiple cleavages, generating Nesfatin-2 (aa 109‑187) and Nesfatin-3 (aa 190‑420). Human Nesfatin-1 shares 85% aa identity with mouse Nesfatin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nesfatin-1/Nucleobindin-2 Antibody (MAB5949)(3)

Publications using MAB5949

• Shuo Zhang, Tao Zhang, Yayun Xu, Genxiang Rong, Juehua Jing Inhibition of NUCB2 suppresses the proliferation, migration, and invasion of rheumatoid arthritis synovial fibroblasts from patients with rheumatoid arthritis in vitro Journal of Orthopaedic Surgery and Research 2022-12-30 [PMID: 36585713] (Western Blot, Human)
• Shuo Zhang, Tao Zhang, Yayun Xu, Genxiang Rong, Juehua Jing Inhibition of NUCB2 suppresses the proliferation, migration, and invasion of rheumatoid arthritis synovial fibroblasts from patients with rheumatoid arthritis in vitro Journal of Orthopaedic Surgery and Research 12/30/2022 [PMID: 36585713]
• L Ren, D Bao, L Wang, Q Xu, Y Xu, Z Shi Nucleobindin-2/nesfatin-1 enhances the cell proliferation, migration, invasion and epithelial-mesenchymal transition in gastric carcinoma Journal of Cellular and Molecular Medicine, 2022-09-06;0(0):. 2022-09-06 [PMID: 36065769] (IHC, Human)

Bioinformatics

• Gene Symbol: NUCB2
• Uniprot:
  • P80303(Human)