Nesfatin-1/Nucleobindin-2 Antibody (877140) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 877140
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 350

Nesfatin-1/Nucleobindin-2 Antibody (877140) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Nesfatin-1/Nucleobindin-2
  Val25-Leu106
  Accession # P80303
• Specificity: Detects human Nesfatin-1/Nucleobindin-2 in direct ELISAs and Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nesfatin-1/Nucleobindin-2 Antibody (877140) [Alexa Fluor® 350]

• DNA-binding protein NEFA
• NEFA
• NEFAGastric cancer antigen Zg4
• Nesfatin1
• Nesfatin-1
• NUCB2
• nucleobindin 2
• Nucleobindin-2
• nucleobinding 2

Background

Nesfatin-1 (NEFA Encoded Satiety and Fat-influencing protein 1) is a secreted, 10 kDa peptide derived from the translation product of the NUCB2 gene. Nesfatin-1 is associated with neurons involved in feeding (ARH and PVH) and fluid intake (SON and PVH), in beta cells of the pancreas, and in adipocytes. Its presence peripherally has an anorexigenic effect. Mature human Nesfatin-1 is 82 amino acids (aa) in length. It is the secreted N-terminus of a larger, intracellular 420 aa precursor termed NEFA/Nucleobindin-2/NUCB2. NUCB2 contains a signal sequence (aa 1‑24), Nesfatin-1 (aa 25‑106), a DNA-binding site (aa 171‑223), and two EF-hand regions (aa 241‑276 and 293‑328). Intracellular uncleaved NUCB2 is 45‑50 kDa in size. There is a potential for multiple cleavages, generating Nesfatin-2 (aa 109‑187) and Nesfatin-3 (aa 190‑420). Human Nesfatin-1 shares 85% aa identity with mouse Nesfatin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.