NELF-E Antibody

Immunohistochemistry-Paraffin: NELF-E Antibody [NBP1-88036] - Staining in human testis and skeletal muscle tissues . Corresponding NELFE RNA-seq data are presented for the same tissues.

Western Blot: NELF-E Antibody [NBP1-88036] - Analysis using Anti-NELFE antibody NBP1-88036 (A) shows similar pattern to independent antibody NBP2-55487 (B).

Western Blot: NELF-E Antibody [NBP1-88036] - Analysis in human cell line MOLT-4.

Immunohistochemistry-Paraffin: NELF-E Antibody [NBP1-88036] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: NELF-E Antibody [NBP1-88036] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Immunohistochemistry-Paraffin: NELF-E Antibody [NBP1-88036] - Staining of human small intestine shows weak to moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: NELF-E Antibody [NBP1-88036] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NELF-E Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESFVSSSDRLRELGPDG
• Predicted Species: Mouse (93%), Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RDBP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  NELF-E Protein (NBP1-88036PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NELF-E Antibody

• D6S45
• negative elongation factor E
• negative elongation factor polypeptide E
• NELFE
• NELF-ERDmajor histocompatibility complex gene RD
• nuclear protein
• RD RNA binding protein
• RD RNA-binding protein
• RDP
• RNA-binding protein RD

Background

NELF-E is encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RDBP