CDAN1 Antibody

Immunohistochemistry-Paraffin: CDAN1 Antibody [NBP2-38473] - Staining in human fallopian tube and skeletal muscle tissues using NBP2-38473 antibody. Corresponding CDAN1 RNA-seq data are presented for the same tissues.

Western Blot: CDAN1 Antibody [NBP2-38473] - Analysis in control (vector only transfected HEK293T lysate) and CDAN1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: CDAN1 Antibody [NBP2-38473] - Staining of human cell line HeLa shows localization to plasma membrane & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: CDAN1 Antibody [NBP2-38473] - Staining of human Fallopian tube shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: CDAN1 Antibody [NBP2-38473] - Staining of human prostate shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: CDAN1 Antibody [NBP2-38473] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: CDAN1 Antibody [NBP2-38473] - Staining of human skin shows moderate cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CDAN1 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: QYRLERGQARRLLHMLLSLWKEDFQGPVPLQLLLSPRNVGLLADTRPREWDLLLFLLRELVEKGLMGRMEIEACLGSLHQAQWPGDFAEELATLSNLFLAEPH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDAN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CDAN1 Protein (NBP2-38473PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CDAN1 Antibody

• CDA1
• CDAI
• CDA-I
• codanin
• codanin-1
• congenital dyserythropoietic anemia, type I
• discs lost homolog
• DLT
• PRO1295

Background

The CDAN1 gene encodes a protein that appears to play a role in nuclear envelope integrity, possibly related to microtubuleattachments. Mutations in this gene cause congenital dyserythropoietic anemia type I, a disease resulting inmorphological and functional

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDAN1
• Uniprot:
  • Q8IWY9(Human)