NEK6 Antibody (SR1470) - BSA Free

Western Blot: NEK6 Antibody (SR1470) [NBP3-22098] - Western blot analysis using NBP3-22098 in HeLa cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clone: SR1470
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NEK6 Antibody (SR1470) - BSA Free Summary

• Description: Novus Biologicals Rabbit NEK6 Antibody (SR1470) - BSA Free (NBP3-22098) is a recombinant monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human NEK6 (Uniprot #: Q9HC98)
• Specificity: Detects endogenous levels of total NEK6
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NEK6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NEK6 Antibody (SR1470) - BSA Free

• EC 2.7.11.1
• Never in mitosis A-related kinase 6
• NIMA (never in mitosis gene a)-related kinase 6
• NimA-related protein kinase 6
• Protein kinase SID6-1512
• putative serine-threonine protein kinase
• serine/threonine-protein kinase Nek6
• SID6-1512

Background

NEK6 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain. The protein is believed to regulate chromatin condensation in mammalian cells via phosphorylation of histones H1 and H3, and it has been shown to phosphorylate ribosomal S6 protein kinase (S6K1) and serum and glucocorticoid induced protein kinase (SGK1), which regulate diverse cellular processes. The protein also has been shown to bind NEK9, another NIMA-related kinase which regulates chromosome alignment and segregation. At least three mRNA transcripts have been identified, 1.6-, 2.6-, and 9.5-kb. The NEK6 gene is localized to 9q33-34, a region at which the loss of heterozygosity is associated with transitional cell carcinomas. NEK6 expression has been documented in many normal human tissues, including blood, brain, heart, liver, lung, skeletal muscle, spleen and testis. ESTs have been isolated from numerous tissue libraries, especially normal cervix and amnion and cancerous bone marrow.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEK6