![Western Blot: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - Wild-type (WT) and NEK7 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25575_rabbit-nek7-mab-hl1348-azide-and-bsa-free-2120249371545.jpg "Western Blot: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - Wild-type (WT) and NEK7 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Ch, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1348 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Rabbit NEK7 Antibody (HL1348) - Azide and BSA Free (NBP3-25575) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NEK7. The exact sequence is proprietary. |
| Source | HEK293 |
| Predicted Species | Rat (100%), Chicken (93%), Rhesus Macaque (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | NEK7 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 35 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for NEK7 Antibody (NBP3-25575)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NEK7 |
![Immunohistochemistry-Paraffin: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. NEK7 stained by NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25575_rabbit-nek7-mab-hl1348-azide-and-bsa-free-2120249342794.jpg "Immunohistochemistry-Paraffin: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. NEK7 stained by NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25575_rabbit-nek7-mab-hl1348-azide-and-bsa-free-2120249355557.jpg "Western Blot: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NEK7 stained by NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25575_rabbit-nek7-mab-hl1348-azide-and-bsa-free-2120249371568.jpg "Immunocytochemistry/Immunofluorescence: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NEK7 stained by NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NEK7 stained by NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25575_rabbit-nek7-mab-hl1348-azide-and-bsa-free-2120249351347.jpg "Immunohistochemistry-Paraffin: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NEK7 stained by NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")