Gelsolin/GSN Antibody


Genetic Strategies: Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with more
Immunocytochemistry/ Immunofluorescence: Gelsolin/GSN Antibody [NBP1-33177] - Methanol-fixed HeLa, using Gelsolin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2000.
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33177] - H1299 xenograft, using Gelsolin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela 7.5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term diluted at 1:500.
Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term diluted at 1:500.
Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000.

Product Details

Reactivity Hu, Mu, Rt, Bv, ShSpecies Glossary
Applications WB, ICC/IF, IHC, KO
Validated by:

Genetic Strategies


Order Details

Gelsolin/GSN Antibody Summary

Recombinant protein encompassing a sequence within the C-terminus region of human Gelsolin. The exact sequence is proprietary.
Isoform 2: Cytoplasm, cytoskeleton, Isoform 1: Secreted
Predicted Species
Sheep (98%), Bovine (98%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
Theoretical MW
86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP1-33177 in the following applications:

  • WB
    2 publications

Reactivity Notes

Chicken (81%).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
0.01% Thimerosal
Immunogen affinity purified

Alternate Names for Gelsolin/GSN Antibody

  • Actin-depolymerizing factor
  • ADF
  • AGEL
  • Brevin
  • DKFZp313L0718
  • gelsolin (amyloidosis, Finnish type)
  • Gelsolin
  • GSN


Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to

Publications for Gelsolin/GSN Antibody (NBP1-33177)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.

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WB Video Protocol
ICC/IF Video Protocol

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Gene Symbol GSN