Uteroglobin/SCGB1A1 Antibody (394324) [Unconjugated]

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Uteroglobin/SCGB1A1 was detected in immersion fixed paraffin-embedded sections of human lung using 15 µg/mL Rat Anti-Human Uteroglobin/SCGB1A1 Monoclonal Antibody (Catalog # MAB4218) overnight at 4 °C. Before ...read more
Uteroglobin/SCGB1A1 was detected in immersion fixed paraffin-embedded sections of human lung using Rat Anti-Human Uteroglobin/SCGB1A1 Monoclonal Antibody (Catalog # MAB4218) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
394324
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Uteroglobin/SCGB1A1 Antibody (394324) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Uteroglobin/SCGB1A1
Glu22-Asn91
Accession # P11684
Specificity
Detects human Uteroglobin/SCGB1A1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
SCGB1A1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB4218 in the following applications:

Publications
Read Publication using
MAB4218 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Uteroglobin/SCGB1A1 Antibody (394324) [Unconjugated]

  • Blastokinin
  • CC10
  • CC10Urine protein 1
  • CC16
  • CCPBP
  • CCSP
  • CCSPCCPBP
  • Clara cell phospholipid-binding protein
  • Clara cells 10 kDa secretory protein
  • Clara
  • PCB-BP
  • SCGB1A1
  • Secretoglobin family 1A member 1
  • secretoglobin, family 1A, member 1 (uteroglobin)
  • UGB
  • UGBUP-1
  • UP1
  • Urinary protein 1
  • Uteroglobin

Background

Uteroglobin, also called Clara cell secretory, phospholipid binding, 10 kDa or 16 kDa protein (CCSP, CCPBP, CC10 or CC16, respectively) is a small, non‑glycosylated secreted protein of the secretoglobin superfamily, (designated 1A, member 1) (1‑3). Its name is derived from its very high expression in the pre‑implantation uterus. It is produced by the non-ciliated, non-mucous secretory cells that predominate in lung bronchioles (Clara cells), and other non-ciliated epithelia that communicate with the external environment (1‑3). Expression is induced by steroid hormones such as estrogen, and enhanced by the non-steroid hormone prolactin (1). Uteroglobin is found in blood, urine and other body fluids (1). Human Uteroglobin cDNA encodes a 21 amino acid (aa) signal sequence and a 70 aa mature protein. It shares 53‑56% aa identity with mouse, rat, bovine, canine, equine or rabbit Uteroglobin, and is active in mice (4). The mature protein forms a disulfide-linked head-to-tail homodimer of 16 kDa (2, 5). This homodimer is thought to form a binding pocket that binds hydrophobic ligands such as phospholipids, progesterone and retinols (5). Sequestering of prostaglandins and leukotrienes is anti-inflammatory, while sequestering of carcinogens such as polychlorinated bisphenols is anti-tumorigenic (6‑8). Other immunoregulatory activities of Uteroglobin include cell migration inhibition (by binding the chemotaxis-related formyl peptide receptor FPR2 on dendritic cells), and the inhibition of T cell differentiation to a Th2 phenotype (9). A single nucleotide polymorphism of Uteroglobin, A38G, confers increased risk of asthma (10). Transglutaminase can crosslink Uteroglobin, either to itself or to other proteins such as the adhesion molecule fibronectin (3, 11). Binding of fibronectin to Uteroglobin in the kidney is thought to protect against nephropathy, while binding of the lipocalin-1 receptor has been reported to suppress cancer cell motility and invasion (12, 13).

  1. Mukherjee, A.B. et al. (2007) Endocr. Rev. 28:707.
  2. Singh, G. et al. (1988) Biochim. Biophys. Acta 950:329.
  3. Mantile, G. et al. (1993) J. Biol. Chem. 268:20343.
  4. Wang, S-Z. et al. (2003) J. Immunol. 171:1051.
  5. Umland, T.C. et al. (1994) Nat. Struct. Biol. 1:538.
  6. Mandal, A.K. et al. (2004) J. Exp. Med. 199:1317.
  7. Watson, T.M. et al. (2001) Am. J. Physiol. Lung Cell Mol. Physiol. 281:L1523.
  8. Yang, Y. et al. (2004) J. Biol. Chem. 279:29336.
  9. Ray, R. et al. (2006) FEBS Lett. 580:6022.
  10. Martin, A.C. et al. (2006) Am. J. Respir. Crit. Care Med. 173:617.
  11. Antico, G. et al. (2006) J. Cell Physiol. 207:553.
  12. Zheng, F. et al. (1999) Nat. Med. 5:1018.
  13. Zhang, Z. et al. (2006) Gene 369:66.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Uteroglobin/SCGB1A1 Antibody (MAB4218)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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Review for Uteroglobin/SCGB1A1 Antibody (MAB4218) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB4218:
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IF
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Uteroglobin/SCGB1A1 MAB4218
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reviewed by:
Veronika Winkelmann
IF Human 11/28/2017
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Summary

ApplicationImmunofluorescence
Sample Testedtracheal epithelial cells
SpeciesHuman
LotYWN021710A

Comments

CommentsCells were fixed with 4% Paraformaldehyde. Human Uteroglobin/SCGB1A1 Antibody was applied at 20 µg/ml at 4°C overnight. Secondary antibody was Goat-anti-Rat-488 (1/400) and Phalloidin-Alexa Fluor 647 (1/50)

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Uteroglobin/SCGB1A1 Antibody (MAB4218). (Showing 1 - 1 of 1 FAQ).

  1. MAB4218 Uteroglobin antibody Can you tell me if it reacts in mouse tissue? 
    • This product has not yet been tested with mouse samples and based on the sequence homology (52%) we do not anticipate this product being able to detect the mouse protein.  If you are looking for a mouse antibody for this target, we have two products that might be better options:https://www.novusbio.com/products/uteroglobin-scgb1a1-antibody-ju34-03_nbp2-75705https://www.novusbio.com/products/uteroglobin-scgb1a1-antibody-122_nbp2-90517

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Recent Reviews

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5
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4
1
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1
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Veronika Winkelmann
11/28/2017
Application: IF
Species: Human

Bioinformatics

Gene Symbol SCGB1A1
Uniprot