NDUFB10 Antibody (OTI1H6) - Azide and BSA Free

Western Blot: NDUFB10 Antibody (OTI1H6) - Azide and BSA Free [NBP2-72915] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB10.

Flow Cytometry: NDUFB10 Antibody (OTI1H6) - Azide and BSA Free [NBP2-72915] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by NDUFB10 antibody.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI1H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

NDUFB10 Antibody (OTI1H6) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human NDUFB10(NP_004539) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NDUFB10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Theoretical MW: 20.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for NDUFB10 Antibody (OTI1H6) - Azide and BSA Free

• CI-PDSW
• complex I PDSW subunit
• Complex I-PDSW
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10 (22kD, PDSW)
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa
• NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
• NADH ubiquinone oxidoreductase PDSW subunit (RH 16p13.3)
• NADH-ubiquinone oxidoreductase PDSW subunit
• PDSW

Background

NDUFB10 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Complex I is composed of 45 different subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFB10