NDRG1 Antibody (4C8) - BSA Free

Immunohistochemistry: NDRG1 Antibody (4C8) [NBP3-26118] - Image of NDRG1 Antibody (4C8) diluted at 1:71.6666666666667 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot: NDRG1 Antibody (4C8) [NBP3-26118] - Positive Western Blot detected in: Hela whole cell lysate, 293T whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate, Mouse kidney tissue. All lanes: NDRG1 Antibody at 0.7ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43, 36, 34 KDa Observed band size: 43 KDa

Immunocytochemistry/Immunofluorescence: NDRG1 Antibody (4C8) [NBP3-26118] - Staining of Hela cells with NDRG1 Antibody (4C8) at 1:23, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4C8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NDRG1 Antibody (4C8) - BSA Free Summary

• Description: Novus Biologicals Rabbit NDRG1 Antibody (4C8) - BSA Free (NBP3-26118) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human NDRG1 [UniProt Q92597]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NDRG1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NDRG1 Antibody (4C8) - BSA Free

• CAP43
• CMT4D
• Differentiation-related gene 1 protein
• DRG1
• DRG-1
• DRG1HMSNL
• GC4
• HMSNL
• NDR1
• NDRG1
• Nickel-specific induction protein Cap43
• NMSL
• N-myc downstream regulated 1
• N-myc downstream-regulated gene 1 protein
• protein NDRG1
• protein regulated by oxygen-1
• PROXY1
• Reducing agents and tunicamycin-responsive protein
• RIT42
• RTP
• RTPGC4
• TARG1
• TDD5
• tunicamycin-responsive protein

Background

NDRG1 is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. Mutation in this gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDRG1