STK38 Antibody (2G8-1F3) - Azide and BSA Free

Western Blot: STK38 Antibody (2G8-1F3) [H00011329-M01] - STK38 monoclonal antibody (M01), clone 2G8-1F3. Analysis of STK38 expression in human kidney.

Immunocytochemistry/Immunofluorescence: STK38 Antibody (2G8-1F3) [H00011329-M01] - Analysis of monoclonal antibody to STK38 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: STK38 Antibody (2G8-1F3) [H00011329-M01] - Analysis of monoclonal antibody to STK38 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue Antibody concentration 5 ug/ml.

Western Blot: STK38 Antibody (2G8-1F3) [H00011329-M01] - STK38 monoclonal antibody (M01), clone 2G8-1F3 Analysis of STK38 expression in Hela S3 NE.

Western Blot: STK38 Antibody (2G8-1F3) [H00011329-M01] - Analysis of STK38 expression in transfected 293T cell line by STK38 monoclonal antibody (M01), clone 2G8-1F3.Lane 1: STK38 transfected lysate(54.2 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: STK38 Antibody (2G8-1F3) [H00011329-M01] - STK38 ( Cat# H00011329-M01 ) on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B. [antibody concentration 3 ug/ml]

Immunoprecipitation: STK38 Antibody (2G8-1F3) [H00011329-M01] - Analysis of STK38 transfected lysate using anti-STK38 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK38 MaxPab rabbit polyclonal antibody.

ELISA: STK38 Antibody (2G8-1F3) [H00011329-M01] - Detection limit for recombinant GST tagged STK38 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 2G8-1F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

STK38 Antibody (2G8-1F3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse STK38 Antibody (2G8-1F3) - Azide and BSA Free (H00011329-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. Anti-STK38 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: STK38 (AAH12085, 1 a.a. ~ 465 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAMTGSTPCSSMSNHTKERVTMTKVTLENFSSNLIAQHEEREMRQKKLEKVMEEEGLKDEEKRLRRSAHARKETEFLRLKRTRLGLEDFESLKVIGRGAFGEVRLVQKKDTGHVYAMKILRKADMLEKEQVGHIRAERDILVEADSLWVVKMFYSFQDKLNLYLIMEFLPGGDMMTLLMKKDTLTEEETQFYIAETVLAIDSIHQLGFIHRDIKPDNLLLDSKGHVKLSDFGLCTGLKKAHRTEFYRNLNHSLPSDFTFQNMNSKRKAETWKRNRRQLAFSTVGTPDYIAPEVFMQTGYNKLCDWWSLGVIMYEMLIGYPPFCSETPQETYKKVMNWKETLTFPPEVPISEKAKDLILRFCCEWEHRIGAPGVEEIKSNSFFEGVDWEHIRERPAAISIEIKSIDDTSNFDEFPESDILKPTVAISNHPETDYKNKDWVFINYTYKRFEGLTARGAIPSYMKAAK
• Localization: Cytoplasmic and Nuclear
• Specificity: STK38 - serine/threonine kinase 38
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: STK38
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactive against recominant protein and cell lysate for Western Blot.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for STK38 Antibody (2G8-1F3) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.1
• Ndr Ser/Thr kinase-like protein
• NDR1 protein kinase
• NDR1
• NDRnuclear Dbf2-related 1
• Nuclear Dbf2-related kinase 1
• serine threonine protein kinase
• serine/threonine kinase 38
• serine/threonine-protein kinase 38

Background

STK38 belongs to the NDR family of serine/threonine protein kinases. NDR kinases require the phosphorylation of conserved Ser/Thr residues for activation. NDR family members have two unique stretches of primary sequence: an N-terminal regulatory (NTR) domain and an insert of several residues between subdomains VII and VIII of the kinase domain. The kinase domain insert functions as an auto-inhibitory sequence (AIS), while binding of the co-activator MOB (Mps-one binder) proteins to the NTR domain releases NDR kinases from inhibition of autophosphorylation. STK38 negatively regulates the activation of MEKK1/2 by direct interaction with the catalytic domain of MEKK1/2. The negative regulation of MEKK1/2 is not due to its phosphorylation by STK38.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STK38 Antibody (H00011329-M01)(1)

Publication using H00011329-M01

• Zhou Y, Adolfs Y, Pijnappel WW et al. MICAL-1 is a Negative Regulator of MST-NDR Kinase Signaling and Apoptosis. Mol Cell Biol. 2011-07-05 [PMID: 21730291]

Bioinformatics

• Gene Symbol: STK38
• Entrez:
  • 11329(Human)
• OMIM:
  • 606964(Human)
• Uniprot:
  • AAH12085(Human)