NDRG1 Antibody (2C6K6)

Western Blot: NDRG1 Antibody (2C6K6) [NBP3-16321] - Western blot analysis of extracts of various cell lines, using NDRG1 Rabbit mAb (NBP3-16321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry: NDRG1 Antibody (2C6K6) [NBP3-16321] - Immunohistochemistry analysis of NDRG1 in paraffin-embedded human colon carcinoma tissue using NDRG1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: NDRG1 Antibody (2C6K6) [NBP3-16321] - Immunohistochemistry analysis of NDRG1 in paraffin-embedded mouse kidney tissue using NDRG1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: NDRG1 Antibody (2C6K6) [NBP3-16321] - Immunohistochemistry analysis of NDRG1 in paraffin-embedded mouse colon tissue using NDRG1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: NDRG1 Antibody (2C6K6) [NBP3-16321] - Immunohistochemistry analysis of NDRG1 in paraffin-embedded human lung squamous carcinoma tissue tissue using NDRG1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: NDRG1 Antibody (2C6K6) [NBP3-16321] - Immunohistochemistry analysis of NDRG1 in paraffin-embedded rat kidney tissue using NDRG1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: NDRG1 Antibody (2C6K6) [NBP3-16321] - Immunohistochemistry analysis of NDRG1 in paraffin-embedded human tonsil tissue using NDRG1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 2C6K6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

NDRG1 Antibody (2C6K6) Summary

• Description: Novus Biologicals Rabbit NDRG1 Antibody (2C6K6) (NBP3-16321) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 295-394 of human NDRG1 (Q92597). ISQPAKLAEAFKYFVQGMGYMPSASMTRLMRSRTASGSSVTSLDGTRSRSHTSEGTRSRSHTSEGTRSRSHTSEGAHLDITPNSGAAGNSAGPKSMEVSC
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NDRG1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NDRG1 Antibody (2C6K6)

• CAP43
• CMT4D
• Differentiation-related gene 1 protein
• DRG1
• DRG-1
• DRG1HMSNL
• GC4
• HMSNL
• NDR1
• NDRG1
• Nickel-specific induction protein Cap43
• NMSL
• N-myc downstream regulated 1
• N-myc downstream-regulated gene 1 protein
• protein NDRG1
• protein regulated by oxygen-1
• PROXY1
• Reducing agents and tunicamycin-responsive protein
• RIT42
• RTP
• RTPGC4
• TARG1
• TDD5
• tunicamycin-responsive protein

Background

NDRG1 is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. Mutation in this gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDRG1