NDP52 Antibody

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Biological Strategies: Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 ...read more
Immunocytochemistry/ Immunofluorescence: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein at autophagosome by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% ...read more
Immunohistochemistry-Paraffin: NDP52 Antibody [NBP2-19499] - Immunohistochemical analysis of paraffin-embedded H441 xenograft, using antibody at 1:100 dilution.
Western Blot: NDP52 Antibody [NBP2-19499] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.
Genetic Strategies: Western Blot: NDP52 Antibody [NBP2-19499] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 ...read more
Biological Strategies: Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. Huh-7 ...read more
Immunoprecipitation: NDP52 Antibody [NBP2-19499] - Jurkat whole cell extracts using 5 ug of NDP52 antibody. Western blot analysis was performed using NDP52 antibody. EasyBlot anti-Rabbit IgG was used as a secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NDP52 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.
Localization
Cytoplasm; perinuclear region; Golgi apparatus; cytoskeleton
Predicted Species
Primate (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CALCOCO2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (97%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for NDP52 Antibody

  • Antigen nuclear dot 52 kDa protein
  • calcium binding and coiled-coil domain 2
  • calcium-binding and coiled-coil domain-containing protein 2
  • NDP52MGC17318
  • Nuclear domain 10 protein 52
  • Nuclear domain 10 protein NDP52
  • Nuclear dot protein 52

Background

The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Understanding Mitophagy Mechanisms: Canonical PINK1/Parkin, LC3-Dependent Piecemeal, and LC3-Independent Mitochondrial Derived Vesicles
By Christina Towers, PhD What is Mitophagy?The selective degradation of mitochondria via double membrane autophagosome vesicles is called mitophagy. Damaged mitochondria can generate harmful amounts of reactive ox...  Read full blog post.

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Bioinformatics

Gene Symbol CALCOCO2
Uniprot