NDP52 Antibody - BSA Free

Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody.The ACTB was used as internal control, shown at the bottom panel. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: NDP52 Antibody [NBP2-19499] - H441 xenograft, using NDP52 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: NDP52 Antibody [NBP2-19499] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. Huh-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody.ACTB was used as internal control, shown at the bottom panel. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunoprecipitation: NDP52 Antibody [NBP2-19499] - Jurkat whole cell extracts using 5 ug of NDP52 antibody. Western blot analysis was performed using NDP52 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Western Blot: NDP52 Antibody [NBP2-19499] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (NBP2-19499) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (NBP2-19499) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NDP52 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NDP52 Antibody - BSA Free (NBP2-19499) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.
• Localization: Cytoplasm; perinuclear region; Golgi apparatus; cytoskeleton
• Predicted Species: Chimpanzee (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CALCOCO2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:10000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for NDP52 Antibody - BSA Free

• Antigen nuclear dot 52 kDa protein
• calcium binding and coiled-coil domain 2
• calcium-binding and coiled-coil domain-containing protein 2
• NDP52MGC17318
• Nuclear domain 10 protein 52
• Nuclear domain 10 protein NDP52
• Nuclear dot protein 52

Background

The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CALCOCO2
• Uniprot:
  • Q13137(Human)