NDC80 Antibody

Western Blot: NDC80 Antibody [H00010403-D01P] - Analysis of NDC80 expression in transfected 293T cell line by NDC80 polyclonal antibody.Lane 1: NDC80 transfected lysate(73.90 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

NDC80 Antibody Summary

• Immunogen: NDC80 (NP_006092.1, 1 a.a. - 642 a.a.) full-length human protein. MKRSSVSSGGAGRLSMQELRSQDVNKQGLYTPQTKEKPTFGKLSINKPTSERKVSLFGKRTSGHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLCEFLTENGYAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKSSMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFLDYTIKCYESFMSGADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIARLEQEREKEPNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE
• Specificity: NDC80 - NDC80 homolog, kinetochore complex component (S. cerevisiae),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDC80
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NDC80 Antibody

• HEC1KNTC2
• HECRetinoblastoma-associated protein HEC
• Highly expressed in cancer protein
• highly expressed in cancer, rich in leucine heptad repeats (yeast)
• highly expressed in cancer, rich in leucine heptad repeats
• hsHec1
• hsNDC80
• kinetochore associated 2
• Kinetochore protein Hec1
• kinetochore protein NDC80 homolog
• Kinetochore-associated protein 2
• NDC80 homolog, kinetochore complex component (S. cerevisiae)
• TID3

Background

HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDC80
• Entrez:
  • 10403(Human)
• Uniprot:
  • NP_006092.1(Human)