NCOR1 Antibody

Images

 
Western Blot: NCOR1 Antibody [NB100-58824] - Whole cell lysate (50 ug) from HEK293T, K-562, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCoR antibodyused for WB at 0.1 ...read more
Chromatin Immunoprecipitation Sequencing: NCOR1 Antibody [NB100-58824] - Localization of NCoR Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-NCoR antibody NB100-58824 and ...read more
Immunoprecipitation: NCOR1 Antibody [NB100-58824] - Detection of Human NCOR on HeLa whole cell lysate using NB100-5882. NCOR was also immunoprecipitated by rabbit anti-NCOR antibody NB100-58825.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IP, CHIP-SEQ
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

NCOR1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1525 and 1575 of human nuclear receptor co-repressor 1 using the numbering given in entry NP_006302.2 (GeneID 9611).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCOR1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 10 ug
  • Immunoprecipitation 2-5 ug/mg lysate
  • Chromatin Immunoprecipitation Sequencing
Application Notes
ChIP-Seq 10 ug as per Ram O et al., Cell. 2011 Dec 23;147(7):1628-39.
Publications
Read Publications using
NB100-58824 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB200-347

Alternate Names for NCOR1 Antibody

  • hN-CoR
  • KIAA1047
  • KIAA1047N-CoR1
  • MGC104216
  • N-CoR
  • NCOR1
  • N-CoRhCIT529I10
  • nuclear receptor corepressor 1
  • nuclear receptor co-repressor 1
  • thyroid hormone- and retinoic acid receptor-associated corepressor 1
  • TRAC1

Background

This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. An alternatively spliced transcript variant has been described, but its full length sequence has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NCOR1 Antibody (NB100-58824)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol

FAQs for NCOR1 Antibody (NB100-58824) (0)

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Secondary Antibodies

 

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Additional NCOR1 Products

Array NB100-58824

Bioinformatics Tool for NCOR1 Antibody (NB100-58824)

Discover related pathways, diseases and genes to NCOR1 Antibody (NB100-58824). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for NCOR1 Antibody (NB100-58824)

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Blogs on NCOR1.

Muscular Dystrophy Regulation: Ezh2, NCoR-1
Over the years muscular dystrophies have become a popular area of research. These are a group of inherited disorders that involve an increase in muscle weakness over time. These disorders greatly decrease the quality of life and there are no known cur...  Read full blog post.

NCOR & EZH2 in Muscular Dystrophy
Over the years muscular dystrophies have become a popular area of research. These are a group of inherited disorders that involve an increase in muscle weakness over time. These disorders greatly decrease the quality of life and there are no known cur...  Read full blog post.

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Bioinformatics

Gene Symbol NCOR1
Entrez
Uniprot