NCOA6 Antibody - BSA Free

Western Blot: NCOA6 Antibody [NB200-336] - Detection of Human ASC2 by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-336 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: NCOA6 Antibody [NB200-336] - Detection of Human ASC2 by Western Blot of Immunoprecipitates

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NCOA6 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NCOA6 Antibody - BSA Free (NB200-336) is a polyclonal antibody validated for use in WB and IP. Anti-NCOA6 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2000 and the C-terminus (residue 2063) of human Activating Signal Cointegrator-2 using the numbering given in Swiss-Prot entry Q14686 (GeneID 23054).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCOA6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-5 ug/mg lysate
  • Western Blot 1:5000-1:10000
• Application Notes: This antibody is useful for Western Blot and Immunoprecipitation. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. *In some cases, the antibody may be diluted further than indicated.
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NCOA6 Antibody - BSA Free

• Activating signal cointegrator 2
• AIB3NRC RAP250
• Amplified in breast cancer protein 3
• ASC-2
• Cancer-amplified transcriptional coactivator ASC-2
• KIAA0181PPAR-interacting protein
• NRCamplified in breast cancer-3 protein
• nuclear receptor coactivator 6
• Nuclear receptor coactivator RAP250
• Nuclear receptor-activating protein, 250 kDa
• peroxisome proliferator-activated receptor interacting protein
• PRIPPeroxisome proliferator-activated receptor-interacting protein
• RAP250Thyroid hormone receptor-binding protein
• thyroid hormone receptor binding protein
• TRBPASC2activating signal cointegrator-2

Background

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development. NCOA6 is over-expressed in several breast cancer cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCOA6 Antibody (NB200-336)(2)

Publications using NB200-336

• Kim GH, Park K, Yeom SY et al. Characterization of ASC-2 as an antiatherogenic transcriptional coactivator of liver X receptors in macrophages. Mol Endocrinol 2009-07-01 [PMID: 19342446]
• Surapureddi S, Rana R, Reddy JK et al. Nuclear receptor coactivator 6 mediates the synergistic activation of human cytochrome P-450 2C9 by the constitutive androstane receptor and hepatic nuclear factor-4alpha. Mol Pharmacol 2008-09-01 [PMID: 18552123]

Bioinformatics

• Gene Symbol: NCOA6
• Uniprot:
  • Q14686(Human)