NCOA3/AIB1 Antibody

Images

 
Western Blot: NCOA3/AIB1 Antibody [NBP1-77785] - Detection of AIB1 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear ...read more
Immunohistochemistry: NCOA3/AIB1 Antibody [NBP1-77785] - Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-NCOA3 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit ...read more
Immunohistochemistry: NCOA3/AIB1 Antibody [NBP1-77785] - Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-NCOA3 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NCOA3/AIB1 Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human AIB1 isoform a protein (NP_858045)
Specificity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with several isoforms of human AIB1 protein (a, b, d, e and f).  A BLAST analysis was used to suggest cross-reactivity with AIB1 from h
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCOA3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:175000
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:100

Reactivity Notes

Reactive in human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NCOA3/AIB1 Antibody

  • ACTR
  • ACTRCBP-interacting protein
  • AIB1
  • AIB1AIB-1
  • Amplified in breast cancer 1 protein
  • BHLHE42
  • bHLHe42p/CIP
  • CAGH16
  • Class E basic helix-loop-helix protein 42
  • CTG26
  • KAT13B
  • MGC141848
  • NCOA3
  • NCoA-3
  • nuclear receptor coactivator 3
  • pCIP
  • pCIP
  • RAC3
  • RAC-3
  • RAC3pCIP
  • Receptor-associated coactivator 3
  • SRC-1
  • SRC3
  • SRC-3
  • SRC-3EC 2.3.1.48
  • Steroid receptor coactivator protein 3
  • Thyroid hormone receptor activator molecule 1
  • TNRC16
  • TRAM1
  • TRAM-1TNRC14

Background

AIB1 /SRC3 (nuclear receptor coactivator 3/steroid receptor coactivator 3 (SRC3)) is a 155 kD member of the p160/steroid receptor coactivator family containing a nuclear localization sequence, bHLH, and PAS domain. There are two reported isoforms of this protein. AIB1 /SRC3 is localized mainly in the cytoplasm, with weak nuclear expression. The protein is translocated to the nucleus upon phosphorylation. AIB1 /SRC3 is widely expressed with high expression in the heart, skeletal muscle, pancreas, and placenta and low expression in the brain. AIB1 /SRC3 is expressed at very low levels in the lung, liver, and kidney and overexpressed in breast and ovarian cancers. This protein has histone acetyltransferase activity and functions as a transcriptional coactivator for steroid and nuclear hormone receptors. Phosphorylation by I kappaB kinase enhances coactivator function. Acetylation by CREB-BP disrupts interaction with nuclear receptors. AIB1 /SRC3 forms a multisubunit coactivation complex with NCOA2 IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein and has also been shown to interact with PCAF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NCOA3
Entrez
Uniprot