NCOA3/AIB1 Antibody - BSA Free

Western Blot: NCOA3/AIB1 Antibody [NBP1-77785] - Detection of AIB1 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kDa, indicated by the arrowhead, corresponds to AIB1. Mouse AIB1(lanes 1 and 4) appear as ~105 kDa bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown). Personal communication, H. Ying, NCI, Bethesda, MD.

Immunohistochemistry: NCOA3/AIB1 Antibody [NBP1-77785] - Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-NCOA3 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry: NCOA3/AIB1 Antibody [NBP1-77785] - Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-NCOA3 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of NCOA3/AIB1 Antibody. Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-NCOA3 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of NCOA3/AIB1 Antibody. Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-NCOA3 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NCOA3/AIB1 Antibody - BSA Free Summary

• Description: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified NCOA3/AIB1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human NCOA3/AIB1/AIB1 isoform a protein. (Uniprot: Q9Y6Q9)
• Specificity: This antibody reacts with several isoforms of human NCOA3/AIB1 protein (a, b, d, e and f).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCOA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:175000
  • Immunohistochemistry 5ug/ml
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:1000
• Application Notes: This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with NCOA3/AIB1 from human and macaque sources based on a 100% homology with the immunizing sequence. Expect partial reactivity with NCOA3/AIB1 from dog, bovine, mouse and rat sources based on partial sequence homology. Cross-reactivity with NCOA3/AIB1 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NCOA3/AIB1 Antibody - BSA Free

• ACTR
• ACTRCBP-interacting protein
• AIB1
• AIB1AIB-1
• Amplified in breast cancer 1 protein
• BHLHE42
• bHLHe42p/CIP
• CAGH16
• Class E basic helix-loop-helix protein 42
• CTG26
• KAT13B
• MGC141848
• NCOA3
• NCoA-3
• nuclear receptor coactivator 3
• pCIP
• pCIP
• RAC3
• RAC-3
• RAC3pCIP
• Receptor-associated coactivator 3
• SRC-1
• SRC3
• SRC-3
• SRC-3EC 2.3.1.48
• Steroid receptor coactivator protein 3
• Thyroid hormone receptor activator molecule 1
• TNRC16
• TRAM1
• TRAM-1TNRC14

Background

AIB1 /SRC3 (nuclear receptor coactivator 3/steroid receptor coactivator 3 (SRC3)) is a 155 kD member of the p160/steroid receptor coactivator family containing a nuclear localization sequence, bHLH, and PAS domain. There are two reported isoforms of this protein. AIB1 /SRC3 is localized mainly in the cytoplasm, with weak nuclear expression. The protein is translocated to the nucleus upon phosphorylation. AIB1 /SRC3 is widely expressed with high expression in the heart, skeletal muscle, pancreas, and placenta and low expression in the brain. AIB1 /SRC3 is expressed at very low levels in the lung, liver, and kidney and overexpressed in breast and ovarian cancers. This protein has histone acetyltransferase activity and functions as a transcriptional coactivator for steroid and nuclear hormone receptors. Phosphorylation by I kappaB kinase enhances coactivator function. Acetylation by CREB-BP disrupts interaction with nuclear receptors. AIB1 /SRC3 forms a multisubunit coactivation complex with NCOA2 IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein and has also been shown to interact with PCAF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCOA3
• Entrez:
  • 8202(Human)
• Uniprot:
  • Q9Y6Q9()
  • Q9Y6Q9()
  • Q9Y6Q9()
  • Q9Y6Q9()
  • Q9Y6Q9()