NCOA3/AIB1 Antibody

Images

 
Independent Antibodies: Western Blot: NCOA3/AIB1 Antibody [NBP1-33720] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NCOA3 antibody [N1N2], N-term.
Immunocytochemistry/ Immunofluorescence: NCOA3/AIB1 Antibody [NBP1-33720] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody [NBP1-33720] - Paraffin-embedded H1299 xenograft, using antibody at 1:500 dilution.
Western Blot: NCOA3/AIB1 Antibody [NBP1-33720] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE, antibody diluted at 1:2000.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

NCOA3/AIB1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human SRC3. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCOA3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for NCOA3/AIB1 Antibody

  • ACTR
  • ACTRCBP-interacting protein
  • AIB1
  • AIB1AIB-1
  • Amplified in breast cancer 1 protein
  • BHLHE42
  • bHLHe42p/CIP
  • CAGH16
  • Class E basic helix-loop-helix protein 42
  • CTG26
  • KAT13B
  • MGC141848
  • NCOA3
  • NCoA-3
  • nuclear receptor coactivator 3
  • pCIP
  • pCIP
  • RAC3
  • RAC-3
  • RAC3pCIP
  • Receptor-associated coactivator 3
  • SRC-1
  • SRC3
  • SRC-3
  • SRC-3EC 2.3.1.48
  • Steroid receptor coactivator protein 3
  • Thyroid hormone receptor activator molecule 1
  • TNRC16
  • TRAM1
  • TRAM-1TNRC14

Background

NCOA3/SRC3 (nuclear receptor coactivator 3/steroid receptor coactivator 3 (SRC3)) is a 155 kD member of the p160/steroid receptor coactivator family containing a nuclear localization sequence, bHLH, and PAS domain. There are two reported isoforms of this protein. NCOA3/SRC3 is localized mainly in the cytoplasm, with weak nuclear expression. The protein is translocated to the nucleus upon phosphorylation. NCOA3/SRC3 is widely expressed with high expression in the heart, skeletal muscle, pancreas, and placenta and low expression in the brain. NCOA3/SRC3 is expressed at very low levels in the lung, liver, and kidney and overexpressed in breast and ovarian cancers. This protein has histone acetyltransferase activity and functions as a transcriptional coactivator for steroid and nuclear hormone receptors. Phosphorylation by I kappaB kinase enhances coactivator function. Acetylation by CREB-BP disrupts interaction with nuclear receptors. NCOA3/SRC3 forms a multisubunit coactivation complex with NCOA2 IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein and has also been shown to interact with PCAF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-310
Species: Hu, Mu, Rt, Mk
Applications: WB, ChIP, IP, IF
NB100-1756
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NB100-381
Species: Hu
Applications: WB, ChIP, IP
NB100-74413
Species: Hu, Mu, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NBP2-47260
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB200-331
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NB100-61050
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB100-328
Species: Hu
Applications: IP (-), WB, IHC, IHC-P, IP
NB100-616
Species: Hu, Mu, Rt, Md, Pm
Applications: WB, ChIP, EM, ICC/IF, IP, In vitro, Flow-IC
NBP1-76729
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB500-487
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P, IP
H00005956-R02
Species: Hu
NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P
AF5415
Species: Hu
Applications: WB, IHC, ICC
NBP1-33720
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for NCOA3/AIB1 Antibody (NBP1-33720) (0)

There are no publications for NCOA3/AIB1 Antibody (NBP1-33720).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NCOA3/AIB1 Antibody (NBP1-33720) (0)

There are no reviews for NCOA3/AIB1 Antibody (NBP1-33720). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NCOA3/AIB1 Antibody (NBP1-33720) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for NCOA3/AIB1 Antibody (NBP1-33720)

Discover related pathways, diseases and genes to NCOA3/AIB1 Antibody (NBP1-33720). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NCOA3/AIB1 Antibody (NBP1-33720)

Discover more about diseases related to NCOA3/AIB1 Antibody (NBP1-33720).
 

Pathways for NCOA3/AIB1 Antibody (NBP1-33720)

View related products by pathway.

PTMs for NCOA3/AIB1 Antibody (NBP1-33720)

Learn more about PTMs related to NCOA3/AIB1 Antibody (NBP1-33720).
 

Research Areas for NCOA3/AIB1 Antibody (NBP1-33720)

Find related products by research area.

Blogs on NCOA3/AIB1

There are no specific blogs for NCOA3/AIB1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NCOA3/AIB1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol NCOA3
Entrez