NCAM-1/CD56 Antibody (ERIC-1) - BSA Free

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, Func, ICC/IF, IHC, IP, CyTOF-ready
Clone
ERIC-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

NCAM-1/CD56 Antibody (ERIC-1) - BSA Free Summary

Immunogen
Retinoblastoma tissue membrane fraction
Localization
Type I membrane protein
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NCAM1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Radioimmunoassay 1:100 - 1:2000
  • Western Blot 1:100 - 1:2000
Application Notes
In the mouse brain, 3 main isoforms have been described of 180,140 and 120 kDa MW. A Western blot of 2 year old pediatric brain revealed bands of 180,140 and 120 kDa (weakly) with ERIC-1. This antibody is CyTOF ready. Positive control(s): Neuroblastome.
Control
NCAM-1/CD56 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for NCAM-1/CD56 Antibody (ERIC-1) - BSA Free

  • CD56 / NCAM-1
  • CD56 antigen
  • CD56
  • MSK39
  • NCAM1
  • N-CAM-1
  • NCAM-1
  • NCAMantigen recognized by monoclonal 5.1H11
  • neural cell adhesion molecule 1
  • neural cell adhesion molecule, NCAM

Background

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NCAM-1/CD56 Antibody (NB100-2718) (0)

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Bioinformatics

Gene Symbol NCAM1
Entrez