NCAM-1/CD56 Antibody (ERIC-1)

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Immunocytochemistry/ Immunofluorescence: NCAM-1/CD56 Antibody (ERIC-1) [NB100-2718] - Double immunofluorescent labeling of NCAM with erythropoietin (EPO) and granzyme B. (A-C) NCAM positive interstitial cells did not ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, RIA, CyTOF-ready
Clone
ERIC-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NCAM-1/CD56 Antibody (ERIC-1) Summary

Immunogen
Retinoblastoma tissue membrane fraction
Localization
Type I membrane protein
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NCAM1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Radioimmunoassay 1:100 - 1:2000
  • CyTOF-ready
Application Notes
In the mouse brain, 3 main isoforms have been described of 180,140 and 120 kDa MW. A Western blot of 2 year old pediatric brain revealed bands of 180,140 and 120 kDa (weakly) with ERIC-1.
Positive Control
NCAM-1/CD56 Lysate (NBL1-13501)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Notes

Positive control(s): Neuroblastome.

Alternate Names for NCAM-1/CD56 Antibody (ERIC-1)

  • CD56 / NCAM-1
  • CD56 antigen
  • CD56
  • MSK39
  • NCAM1
  • N-CAM-1
  • NCAM-1
  • NCAMantigen recognized by monoclonal 5.1H11
  • neural cell adhesion molecule 1
  • neural cell adhesion molecule, NCAM

Background

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NCAM-1/CD56 Antibody (NB100-2718) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NCAM1
Entrez