NCAM-1/CD56 Antibody (CL12732) - Azide and BSA Free

Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-44370 antibody. Corresponding NCAM-1/CD56 RNA-seq data are presented for the same tissues.

Staining of human heart shows moderate positivity in the intercalated discs in cardiomyocytes.

Staining of human duodenum shows strong positivity in peripheral ganglion and nerves.

Staining of human cerebral cortex shows strong positivity in neuropil.

Staining of human malignant glioma shows strong positivity in tumor cells.

Staining of human malignant melanoma shows moderate membranous positivity in tumor cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human ovarian cancer (papillary serous cystadenocarcinoma) shows strong membranous positivity in tumor cells.

• Reactivity: Hu
• Applications: IHC
• Clone: CL12732
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

NCAM-1/CD56 Antibody (CL12732) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse NCAM-1/CD56 Antibody (CL12732) - Azide and BSA Free (NBP3-18543) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P13591, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NCAM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
• Application Notes: For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for NCAM-1/CD56 Antibody (CL12732) - Azide and BSA Free

• CD56 / NCAM-1
• CD56 antigen
• CD56
• MSK39
• NCAM1
• N-CAM-1
• NCAM-1
• NCAMantigen recognized by monoclonal 5.1H11
• neural cell adhesion molecule 1
• neural cell adhesion molecule, NCAM

Background

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCAM1