NCAM-1/CD56 Antibody (CL12732) - BSA Free

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (CL12732) [NBP3-18543] - Staining of human heart shows moderate positivity in the intercalated discs in cardiomyocytes.

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (CL12732) [NBP3-18543] - Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-18543 antibody. Corresponding NCAM1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (CL12732) [NBP3-18543] - Staining of human ovarian cancer (papillary serous cystadenocarcinoma) shows strong membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (CL12732) [NBP3-18543] - Staining of human malignant glioma shows strong positivity in tumor cells.

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (CL12732) [NBP3-18543] - Staining of human malignant melanoma shows moderate membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (CL12732) [NBP3-18543] - Staining of human cerebral cortex shows strong positivity in neuropil.

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (CL12732) [NBP3-18543] - Staining of human duodenum shows strong positivity in peripheral ganglion and nerves.

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (CL12732) [NBP3-18543] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: CL12732
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

NCAM-1/CD56 Antibody (CL12732) - BSA Free Summary

• Description: Novus Biologicals Mouse NCAM-1/CD56 Antibody (CL12732) - BSA Free (NBP3-18543) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from P13591, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NCAM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
• Application Notes: For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for NCAM-1/CD56 Antibody (CL12732) - BSA Free

• CD56 / NCAM-1
• CD56 antigen
• CD56
• MSK39
• NCAM1
• N-CAM-1
• NCAM-1
• NCAMantigen recognized by monoclonal 5.1H11
• neural cell adhesion molecule 1
• neural cell adhesion molecule, NCAM

Background

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCAM1