NCAM-1/CD56 Antibody (123C3.D5)

Images

 
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis of NCAM-1/CD56 antibody (123C3.D5) on Human colon tissue. Incubation: 1 ug/ml for 30 minutes at room temperature. Image from ...read more
Flow (Cell Surface): NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - A surface stain was performed on U-87 Cells with NCAM-1/CD56 Antibody (123C3) NBP2-33132B and a matched isotype control. Both antibodies were ...read more
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Formalin-fixed, paraffin-embedded human colon Ganglion stained with CD56 Monoclonal Antibody (123C3.D5)
Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - analysis of CD56 on human monocytes using CD56 antibody at 1 ug/10^6 cells.
Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis using the Azide and BSA Free version of NBP2-15184. Staining of AF700 conjugated CD56 in human PBMC using anti-CD56 antibody. The primary antibody ...read more
Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Flow Cytometry: NCAM-1/CD56 Antibody (123C3 (same as 123C3.D5)) [Alexa Fluor® 647] [NBP2-33132AF647] - A surface stain was performed on SH-SY5Y cells ...read more
Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Simple Western lane view shows a specific band for NCAM-1/CD56 in 0.2 mg/ml of IMR-32 lysate(s). This experiment was performed under reducing conditions ...read more
Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Electropherogram image of the corresponding Simple Western lane. NCAM-1/CD56 antibody was used at 10 ug/ml dilution of IMR-32 lysates(s) respectively.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications Simple Western, Flow, Flow-CS, ICC/IF, IHC, IHC-P
Clone
123C3.D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

NCAM-1/CD56 Antibody (123C3.D5) Summary

Immunogen
Membrane preparation of a small cell lung carcinoma
Localization
Cell surface
Marker
Neuronal Cell Marker
Specificity
This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NCAM1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Cell Surface)
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Simple Western 10 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Reviewed Applications
Read 2 Reviews rated 4
using
NBP2-15184 in the following applications:

Publications
Read Publications using
NBP2-15184 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33132)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for NCAM-1/CD56 Antibody (123C3.D5)

  • CD56 / NCAM-1
  • CD56 antigen
  • CD56
  • MSK39
  • NCAM1
  • N-CAM-1
  • NCAM-1
  • NCAMantigen recognized by monoclonal 5.1H11
  • neural cell adhesion molecule 1
  • neural cell adhesion molecule, NCAM

Background

CD56, a 175-220KDa glycoprotein, is a member of the Ig super family. It is expressed as three major isoforms and consists of five Ig-like domains and two Fibronectin-type III domains in the extracellular region. The 135kDa isoform is the basic molecule which is glycosylated or sialylated to produce the mature species. CD56 is widely expressed in nervous system, on NK cells and a specific set of Tcells. CD56+ NK cells and Tcells are unique in their ability to mediate cell-mediated cytotoxicity against certain tumor cell targets without MHC restriction. Other physiological functions of CD56 include mediating cell adhesion through homophilic and heterophilic interaction and activating intracellular signaling pathways resulting in neutrite extension and fasciculation, migration and synapses formation in brain. CD56 is also vital for neuronal development and plasticity in adult brain. It is used as a tumor marker in various cancers such as NK lymphomas and Merkel cell carcinoma. NCAM is expressed on most neuroectodermal derived lines, tissues, and neoplasms such as retinoblastoma, medulloblastoma, astrocytoma, and neuroblastoma. It is also expressed on some mesodermally derived tumors such as rhabdomyosarcoma and also on natural killer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NCAM-1/CD56 Antibody (NBP2-15184)(6)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Rat
(2)
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Reviews for NCAM-1/CD56 Antibody (NBP2-15184) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-15184:
Filter by Applications
IHC-P
(1)
IF
(1)
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Filter by Species
Human
(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin NCAM-1/CD56 NBP2-15184
Enlarge
5
reviewed by:
Verified Customer
IHC-P Human 02/12/2022
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedColon tissue
SpeciesHuman
Lot123C3.D5

Comments

CommentsIncubation: 1 ug/ml for 30 minutes at room temperature.
  3
reviewed by:
Verified Customer
IF Mouse 09/21/2017
View

Summary

ApplicationImmunofluorescence
Sample TestedCortical neurons
SpeciesMouse
Lot4684-1P161212

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ICC/IF Video Protocol

FAQs for NCAM-1/CD56 Antibody (NBP2-15184) (0)

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Secondary Antibodies

 

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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

Verified Customer
02/12/2022
Application: IHC-P
Species: Human

 
Verified Customer
09/21/2017
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol NCAM1
Uniprot