Nbs1 Antibody [Alexa Fluor® 594]

• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 594

Nbs1 Antibody [Alexa Fluor® 594] Summary

• Immunogen: Phosphopeptide containing human Nbs1 S343 site
• Specificity: Detects human Nbs1 when phosphorylated at S343. Does not recognize Nbs1 when unphosphorylated at S343.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

The Nijmegen Breakage Syndrome 1 (Nbs1) protein is a member of the Mre11/Rad50/Nbs1 (MRN) protein complex that binds to DNA double-strand breaks in cells exposed to DNA damaging agents. In addition, the MRN complex colocalizes with replication forks during DNA replication. The MRN complex plays an important role in routine cell cycle progression and genotoxic stress responses by facilitating DNA repair. Nbs1 is phosphorylated at S343 by ATM in response to double-strand breaks and by ATR under replication stress. This phosphorylation triggers the inactivation of late origin firing, which is essential for mediating the intra-S-phase checkpoint. Mutation of the nbs1 gene and resultant loss of Nbs1 protein expression in humans results in the chromosomal instability disease, Nijmegen Breakage Syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Nbs1 Antibody (AF4944T). (Showing 1 - 4 of 4 FAQ).

1. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
   • Yes, if this product is used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product.
2. What research areas can this product be used in?
   • All Nbs1 products can be used in Breast Cancer, Cancer, Checkpoint signaling, Chromatin Research, DNA Double Strand Break Repair, DNA Repair, Homologous Recombination, Non-homologous end-joining, Tumor Suppressors, and Cell Cycle and Replication.
3. What is NBS1?
   • NBS1 is Nijmegen breakage syndrome protein 1, or Nibrin. Please refer to the background listed in the datasheet for a full summary of this common name.
4. What is the theoretical molecular weight of Nbs1 antibodies?
   • In general, there is only one isoform described for this target. The TMW of the human form of the target protein is 85kDa. The mouse form is 84kDa. However, the observed molecular weight may vary.