NAPRT1 Antibody

Western Blot: NAPRT1 Antibody [NBP2-19471] - A: HeLa.

Immunocytochemistry/Immunofluorescence: NAPRT1 Antibody [NBP2-19471] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using NAPRT1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP2-19471] - Immunohistochemical analysis of paraffin-embedded Lung ca, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NAPRT1 Antibody Summary

• Description: Novus Biologicals Rabbit NAPRT1 Antibody (NBP2-19471) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NAPRT1. The exact sequence is proprietary.
• Localization: Cytoplasm; cytosol
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NAPRT
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for NAPRT1 Antibody

• EC 2.4.2.11
• FHA-HIT-interacting protein
• FHIP
• NAPRTase
• nicotinate phosphoribosyltransferase domain containing 1
• Nicotinate phosphoribosyltransferase domain-containing protein 1
• nicotinate phosphoribosyltransferase
• nicotinic acid phosphoribosyltransferase
• PP3856

Background

Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NAPRT