ALDH1L1 Antibody (2E7) - BSA Free

Western Blot: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of different tissue and cell lysates using mouse mAb to ALDH1L1, NBP2-50033, dilution 1:5,000 in green: [1] protein standard (red), rat tissue lysates: [2] heart, [3] liver, [4] kidney,[5] lung, [6] brain, and [7] spinal cord; mouse tissue lysates: [8] brain, and [9] spinal cord; cell lysates: [10] NIH-3T3, and [11] HEK293. The band at 100kDa mark corresponds to ALDH1L1 protein.

Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to aldehyde dehydrogenase 1L1 (ALDH1L1), NBP2-50033, dilution 1:1,000 in red, and costained with chicken pAb to GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. NBP2-50033 antibody produces cytoplasmic staining of glial cells, while the GFAP antibody labels the intermediate filament cytoskeleton in astrocytes and other glial cells. Some astrocytic cells express both ALDH1L1 and GFAP and appear yellow.

Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033] - Neuron-glia cell mixed cultures stained with NBP2-50033 (red) and chicken polyclonal antibody against Vimentin (NB300-223, green). Blue is a DNA stain. NBP2-50033 stains astrocytic cell bodies and processes. The fibroblastic cells contain only Vimentin and so are green, while astrocytes contain either Vimentin and ALDH1L1, so appearing golden, or predominantly ALDH1L1, in which case they appear red.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF, IHC
• Clone: 2E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ALDH1L1 Antibody (2E7) - BSA Free Summary

• Description: Novus Biologicals Mouse ALDH1L1 Antibody (2E7) - BSA Free (NBP2-50033) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Amino acids 402-902 of human ALDH1L1. [UniProt# O75891]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH1L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Simple Western 1:20 - 1:250
  • Western Blot 1:5000 - 1:10000
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in Mouse brain lysate, separated by Size, antibody dilution of 1;20, 1:100, 1:250, apparent MW was 91 kDa
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 0.035% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ALDH1L1 Antibody (2E7) - BSA Free

• 10-formyltetrahydrofolate dehydrogenase
• aldehyde dehydrogenase 1 family, member L1
• aldehyde dehydrogenase family 1 member L1
• Cytosolic 10-formyltetrahydrofolate dehydrogenase
• DKFZp781N0997
• EC 1.5.1.6,10-FTHFDH
• FDH
• formyltetrahydrofolate dehydrogenase
• FTHFD10-fTHF

Background

ALDH1L1 is encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALDH1L1 Antibody (NBP2-50033)(3)

Publications using NBP2-50033

• Schmitt EC The Role of AKT2 in Astrogliosis & Behavior and Nicotinic Regulation of CYP2A5 Thesis 2021-01-01
• Milstead R, Link C, Xu Z, Hoeffer C Molecular Signaling and Neural Cell Type Expression of Complex Brain Disorders Thesis Jul 13 1905 (IHC-FrFl, Mouse)
• Milstead R, Link C, Xu Z, Hoeffer C Molecular Signaling and Neural Cell Type Expression of Complex Brain Disorders Thesis 1905-07-13 (IHC-FrFl, Mouse)

Review for ALDH1L1 Antibody (NBP2-50033) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 07/19/2017

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: PFA-fixed cryosections of mouse brain
• Species: Mouse
• Lot: 102113

Comments

• Comments: Only works after antigen retrieval (citrate buffer, pH6; (sub)boiling for 10 min)
  Dilution 1:100

Bioinformatics

• Gene Symbol: ALDH1L1
• Uniprot:
  • O75891(Human)