Nanog Antibody

Images

 
Western Blot: Nanog Antibody [NBP1-77109] - Human spleen tissue lysate antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NBP1-77109] - Immunofluorescence of NANOG in Human Spleen cells with NANOG antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: Nanog Antibody [NBP1-77109] - Human spleen tissue with NANOG antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Nanog Antibody Summary

Immunogen
Antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG. The immunogen is located within amino acids 130 - 180 of NANOG.
Marker
Embryonic Stem Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NANOG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Nanog Peptide (NBP1-77109PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nanog Antibody

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox
  • Nanog

Background

Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nanog Antibody (NBP1-77109) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nanog Antibody (NBP1-77109). (Showing 1 - 1 of 1 FAQ).

  1. As part of an Endometriosis-related project in horses, I would like to ask some information with regard to this. I would like to know about its tested or predicted reactivity with horse.
    • This antibody has not yet been tested with horse. This means that use of this antibody with horse would not be covered by our guarantee; however, you would be eligible for our Innovator's Reward Program. For an indication of the likely cross-reactivity (how well the antibody is likely to perform with horse), I have run UniProt Blast sequence comparison of the immunogen sequence versus horse proteins. For Nanog Antibody NBP1-77109, the exact immunogen sequence is not available, so I have had to use the whole of the human protein, database reference EAW88651, for the sequence analysis. The result was a 76% match to F6PXN9 and a 75% match to F6PPY5. Unfortunately these proteins from Equus caballus (horse) are unreviewed.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Nanog Antibody (NBP1-77109)

Discover related pathways, diseases and genes to Nanog Antibody (NBP1-77109). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Nanog Antibody (NBP1-77109)

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Blogs on Nanog.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

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Bioinformatics

Gene Symbol NANOG
Entrez
Uniprot