NACA1 Antibody - Azide and BSA Free

Western Blot: NACA1 Antibody [H00004666-B01P] - Analysis of NACA expression in transfected 293T cell line by NACA polyclonal antibody. Lane 1: NACA transfected lysate(23.65 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: NACA1 Antibody [H00004666-B01P] - Analysis of purified antibody to NACA on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: NACA1 Antibody [H00004666-B01P] - analysis of NACA expression in transfected 293T cell line by NACA MaxPab polyclonal antibody. Lane 1: NACA transfected lysate (23.65 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NACA1 Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: NACA (NP_005585.1, 1 a.a. - 215 a.a.) full-length human protein. MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQATTQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM
• Specificity: NACA - nascent polypeptide-associated complex alpha subunit,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: NACA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NACA1 Antibody - Azide and BSA Free

• Allergen Hom s 2
• alpha-NAC
• MGC117224
• NACA1
• NAC-alpha
• nascent polypeptide-associated complex alpha subunit
• nascent polypeptide-associated complex subunit alpha
• nascent-polypeptide-associated complex alpha polypeptide

Background

Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NACA1 Antibody (H00004666-B01P)(2)

Publications using H00004666-B01P

• Alice Z, Aikaterini T, Chrysoula D et al. The nascent polypeptide-associated complex (NAC) controls translation initiation in cis by recruiting nucleolin to the encoding mRNA. Nucleic Acids Res. 2022-09-23 [PMID: 36107769]
• Puigmule M, Lopez-Hellin J, Sune G et al. Differential proteomic analysis of cyclosporine A-induced toxicity in renal proximal tubule cells . Nephrol Dial Transplant. 2009-04-15 [PMID: 19369687]

Bioinformatics

• Gene Symbol: NACA
• Entrez:
  • 4666(Human)