Myosin Heavy Chain Antibody (MF20) [CoraFluor™ 1]

• Reactivity: All-Multi
• Applications: WB, Flow, IHC, ICC/IF
• Clone: MF20
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Myosin Heavy Chain Antibody (MF20) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Chicken pectoralis-derived Myosin
• Specificity: Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYH14
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Myosin Heavy Chain Antibody (MF20) [CoraFluor™ 1]

• DFNA4
• FLJ13881
• FLJ43092
• KIAA2034DKFZp667A1311
• MHC16
• MYH14 variant protein
• MYH17
• MYHC
• Myosin heavy chain 14
• Myosin Heavy Chain
• Myosin heavy chain, non-muscle IIc
• myosin
• myosin, heavy chain 14
• myosin, heavy chain 14, non-muscle
• myosin, heavy polypeptide 14
• myosin-14
• NMHC II-C
• NMHC-II-C
• Non-muscle myosin heavy chain IIc
• nonmuscle myosin heavy chain II-C

Background

Myosin heavy chain is a muscle myosin, which is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Cardiac myosin heavy chain exists as two isoforms in humans, alpha-cardiac MHC and beta-cardiac MHC. These two isoforms are expressed in different amounts in the human heart. During normal physiology, beta-cardiac MHC is the predominant form, with the alpha-isoform contributing around only 7% of the total MHC. Mutations of the myosin heavy chain genes are associated with several different dilated and hypertrophic cardiomyopathies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYH14