Myosin Heavy Chain Antibody (MF20) [Alexa Fluor® 647]

• Reactivity: All-Multi
• Applications: WB, Flow, IHC, ICC/IF
• Clone: MF20
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Myosin Heavy Chain Antibody (MF20) [Alexa Fluor® 647] Summary

• Immunogen: Chicken pectoralis-derived Myosin
• Specificity: Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myosin Heavy Chain Antibody (MF20) [Alexa Fluor® 647]

• DFNA4
• FLJ13881
• FLJ43092
• KIAA2034DKFZp667A1311
• MHC16
• MYH14 variant protein
• MYH17
• MYHC
• Myosin heavy chain 14
• Myosin Heavy Chain
• Myosin heavy chain, non-muscle IIc
• myosin
• myosin, heavy chain 14
• myosin, heavy chain 14, non-muscle
• myosin, heavy polypeptide 14
• myosin-14
• NMHC II-C
• NMHC-II-C
• Non-muscle myosin heavy chain IIc
• nonmuscle myosin heavy chain II-C

Background

Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.