Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A))

Images

 
Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using ...read more
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Sodium Potassium ATPase Beta 1 antibody was tested in HepG2 cells with Dylight 488 (green). ...read more
Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Detection of Na, K-ATPase (beta) in rat kidney homogenates (20 ug)using NB 300-147. Lane 1: 1:2,500 primary. Lane 2: ...read more

Product Details

Summary
Reactivity Hu, Rt, Po, Bv, Ca, Ch, Ha, Rb, Xp, Mu(-)Species Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
464.8 (also known as 8A)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) Summary

Immunogen
Purified Sodium Potassium ATPase Beta 1 from rabbit renal outer medulla. [UniProt# Q9TT37]
Marker
Plasma Membrane Marker
Specificity
This is specific for Na,K-ATPase beta 1 subunit.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ATP1B1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-1:5000
Application Notes
This Sodium Potassium ATPase Beta 1 (464.8) antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. Immunohistochemistry-Paraffin was reported in scientific literature. This antibody may have a broad banding pattern, typical of glycosylated proteins, at ~35-40 kDa. One may see multiple bands migrating between 35-65 kDa. Do not boil the sample prior to loading on the gel for Western Blot (60 degrees Celsius appears to work fine).
Control
Sodium Potassium ATPase Beta 1 Overexpression Lysate
Publications
Read Publications using
NB300-147 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    11 publications

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 31356813).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A))

  • adenosinetriphosphatase
  • ATP1B
  • ATP1B1
  • ATPase, Na+/K+ transporting, beta 1 polypeptide
  • Beta 1-subunit of Na(+)
  • K(+)-ATPase
  • MGC1798
  • Na, K-ATPase beta-1 polypeptide
  • Sodium Potassium ATPase Beta 1
  • sodium/potassium-dependent ATPase beta-1 subunit
  • Sodium/potassium-dependent ATPase subunit beta-1
  • sodium/potassium-transporting ATPase beta-1 chain
  • sodium/potassium-transporting ATPase subunit beta-1

Background

Sodium Potassium ATPase Beta 1 (NKA-beta1), member of X(+)/potassium ATPases subunit beta family, is a single-pass type II membrane protein (transmembrane enzyme) responsible for active reciprocal transport of Na+/K+ ions across plasma membranes, and it contains a larger alpha subunit which catalyze ATP hydrolysis and a smaller beta subunit which is the non-catalytic component of the active enzyme. The alpha subunit (113 kD) contains the binding sites for ATP, Na+ ions, K+ ions, and allosteric sites for inhibitors and activators, and is capable of catalyzing ATP hydrolysis as an essential energy source to transport 3 Na+ ions out of the cell and 2 K+ ions into the cell against membrane ion gradients. The beta subunit (35 kD) is a glycoprotein with isoforms beta1, beta2 and beta3 which are non-catalytic in nature because they lack binding sites for ATP or Na+/K+ ions. In fact, beta subunit is a specific chaperone that assists the biogenesis and corrects membrane insertion of newly synthesized NKA. Moreover, it exerts allosteric activities and by altering the apparent affinity of Na+ /K+ ions as well as by enhancing enzyme turnover rate, NKA-beta1 remotely controls the catalytic function of the enzyme through JY2948-beta1-alpha1 interaction complex. Furthermore, NKA-beta1 may dictate functional NKA concentrations in caveolae, can promote translation efficiency of the alpha subunit and mediate alpha/beta assembly as well as beta-beta homo-oligomerization, and is essential to epithelial polarization, suppression of invasion, and cell motility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Sodium Potassium ATPase Beta 1 Antibody (NB300-147)(15)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Canine, Chicken, Porcine, Rabbit, Xenopus.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-P
(1)
WB
(11)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
Rat
(2)
Canine
(3)
Chicken
(1)
Porcine
(2)
Rabbit
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB300-147 Applications Species
Sampaio L, Shiju T, Hilgert G Et al. Descemet's membrane injury and regeneration, and posterior corneal fibrosis in rabbits Experimental Eye Research Nov 1 2021 [PMID: 34736886] (IHC, Rabbit) IHC Rabbit
Soltysik K, Ohsaki Y, Tatematsu T et al. Nuclear lipid droplets form in the inner nuclear membrane in a seipin-independent manner The Journal of cell biology Jan 4 2021 [PMID: 33315072]
Shimamoto S, Nakashima K, Kamimura R et al. Insulin acutely increases glucose transporter 1 on plasma membranes and glucose uptake in an AKT-dependent manner in chicken adipocytes Gen. Comp. Endocrinol. Jul 26 2019 [PMID: 31356813] (WB, Chicken) WB Chicken
Tokhtaeva E, Sun H, Deiss-Yehiely N et al. FXYD5 O-glycosylated ectodomain impairs adhesion by disrupting cell-cell trans-dimerization of Na,k-ATPase beta1 subunits. J. Cell. Sci. May 3 2016 [PMID: 27142834] (WB, Human) WB Human
Tokhtaeva E, Capri J, Marcus EA et al. Septin dynamics are essential for exocytosis J. Biol. Chem. 2015 Jan 09 [PMID: 25575596] (WB, Human) WB Human
Carmosino Monica, Torretta Silvia, Procino Giuseppe et al. Na+/K+-ATPase beta1-subunit is recruited in Na-K-2Cl co-transporter isoform 2 multiprotein complexes in rat kidneys: possible role in blood pressure regulation. J Hypertens. 2014 Sep 01 [PMID: 24927069] (Rat) Rat
Gong H, Sun J, Xue W et al. Protective effect of truncated Na+/K+-ATPase beta on ischemia/reperfusion-induced renal injury in rats. Exp. Biol. Med. (Maywood) 2014 Apr 15 [PMID: 24737734] (WB, Human) WB Human
Tokhtaeva E, Sachs G, Sun H et al. Identification of the amino acid region involved in the intercellular interaction between the beta 1 subunits of Na/K-ATPase J Cell Sci 2012 Mar 15 [PMID: 22328500] (WB, Canine, Rat) WB Canine, Rat
Tang WH, Stitham J, Gleim S et al. Glucose and collagen regulate human platelet activity through aldose reductase induction of thromboxane. J Clin Invest 2011 Nov [PMID: 22005299] (Human) Human
Mastrodonato M, Calamita G, Rossi R et al. Expression of H+,K+-ATPase Glycopattern Analysis in the Gastric Gls of Rana esculenta. J Histochem Cytochem;57(3):215-225. 2009 [PMID: 19001639] (IHC-P, WB, Xenopus) IHC-P, WB Xenopus
Show All 15 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ATP1B1
Entrez
Uniprot