Myosin Heavy Chain Antibody (MF20) [Alexa Fluor® 700]

• Applications: WB, Flow, IHC, ICC/IF
• Clone: MF20
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Myosin Heavy Chain Antibody (MF20) [Alexa Fluor® 700] Summary

• Immunogen: Chicken pectoralis-derived Myosin
• Specificity: Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.