Pax7 Antibody (PAX7) [Unconjugated]

Pax7 was detected in immersion fixed C2C12 mouse myoblast cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) for 3 hours at room temperature. Cells were stained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Pax7 was detected in immersion fixed iBJ6 iPS cell line differentiated into myoblasts using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Pax7 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: IHC, ICC/IF
• Clone: PAX7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Pax7 Antibody (PAX7) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant chicken Pax7
  aa 352-523
• Specificity: Detects human, mouse, rat, and chicken Pax7 (3-8).
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAX7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax7 Antibody (PAX7) [Unconjugated]

• FLJ37460
• HUP1
• paired box 7
• paired box gene 7
• paired box homeotic gene 7
• paired box protein Pax-7
• paired domain gene 7
• PAX7 transcriptional factor
• Pax7
• PAX7B
• RMS2

Background

Pax7 belongs to the paired box protein family of transcription factors. It is an important regulator of neural and skeletal muscle development (1 - 3).

1. Jostes, B. et al. (1990) Mech. Dev. 33(1):27.
2. Asakura, A. et al. (2001) Differentiation 68(4-5):245.
3. Kawakami, A. et al. (1997) Mech. Dev. 66:119.
4. Ericson, J. et al. (1996) Cell 87:661.
5. Berggren, K. et al. (2001) Dev. Dyn. 222:1.
6. Takebayashi, H. et al. (2002) Mech. Dev. 113:169.
7. McLoon, L.K. and J. Wirtschafter (2003) Invest. Opthamal. Vis. Sci. 44:1927.
8. Yamamoto, S. et al. (2001) J. Neurosci. 21:9814.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pax7 Antibody (MAB1675)(48)

Publications using MAB1675

• Lim, C;McKendry, J;Giacomin, T;Mcleod, JC;Ng, SY;Currier, BS;Coletta, G;Phillips, SM; Fortetropin supplementation prevents the rise in circulating myostatin but not disuse-induced muscle atrophy in young men with limb immobilization: A randomized controlled trial PloS one 2023-05-23 [PMID: 37220119] (IHC, Human)
• Y Cao, J Tan, H Zhao, T Deng, Y Hu, J Zeng, J Li, Y Cheng, J Tang, Z Hu, K Hu, B Xu, Z Wang, Y Wu, PE Lobie, S Ma Bead-jet printing enabled sparse mesenchymal stem cell patterning augments skeletal muscle and hair follicle regeneration Nature Communications, 2022-12-03;13(1):7463. 2022-12-03 [PMID: 36460667] (Immunohistochemistry, Mouse)
• T Zhang, C Künne, D Ding, S Günther, X Guo, Y Zhou, X Yuan, T Braun Replication collisions induced by de-repressed S-phase transcription are connected with malignant transformation of adult stem cells Nature Communications, 2022-11-14;13(1):6907. 2022-11-14 [PMID: 36376321] (ICC, Mouse)
• Yamakawa D, Tsuboi J, Kasahara K et al. Cilia-Mediated Insulin/Akt and ST2/JNK Signaling Pathways Regulate the Recovery of Muscle Injury Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11/14/2022 [PMID: 36373718]
  
  Details:
  Dilution used in WB 1:1000
• David W. Hammers NOX4 inhibition promotes the remodeling of dystrophic muscle JCI Insight 2022-10-24 [PMID: 36278481]
• I Kim, A Ghosh, N Bundschuh, L Hinte, E Petrosyan, F von Meyenn, O Bar-Nur Integrative molecular roadmap for direct conversion of fibroblasts into myocytes and myogenic progenitor cells Science Advances, 2022-04-06;8(14):eabj4928. 2022-04-06 [PMID: 35385316] (ICC/IF, Mouse)
• Sílvia Gras, Alba Blasco, Guillem Mòdol-Caballero, Olga Tarabal, Anna Casanovas, Lídia Piedrafita et al. Beneficial effects of dietary supplementation with green tea catechins and cocoa flavanols on aging-related regressive changes in the mouse neuromuscular system Aging (Albany NY) 2021-07-31 [PMID: 34319911]
• C Gong, X Zheng, F Guo, Y Wang, S Zhang, J Chen, X Sun, SZA Shah, Y Zheng, X Li, Y Yin, Q Li, X Huang, T Guo, X Han, SC Zhang, W Wang, H Chen Human spinal GABA neurons alleviate spasticity and improve locomotion in rats with spinal cord injury Cell Reports, 2021-03-23;34(12):108889. 2021-03-23 [PMID: 33761348] (ICC, Rat)
• Mario Parstorfer, Francesca Profit, Nadine Weiberg, Michaela Wehrstein, Alexander Barié, Birgit Friedmann-Bette Increased satellite cell apoptosis in vastus lateralis muscle after anterior cruciate ligament reconstruction Journal of Rehabilitation Medicine 2021-02-17 [PMID: 33569607]
• Meng-Huang Wu, Chuang-Yu Lin, Chun-Yin Hou, Ming-Thau Sheu, Hsi Chang Micronized sacchachitin promotes satellite cell proliferation through TAK1-JNK-AP-1 signaling pathway predominantly by TLR2 activation Chinese Medicine 2020-12-01 [PMID: 33514380]
• Christian Schutt, Alix Hallmann, Salma Hachim, Ina Klockner, Melissa Valussi, Ann Atzberger et al. Linc‐ MYH configures INO 80 to regulate muscle stem cell numbers and skeletal muscle hypertrophy The EMBO Journal 2020-11-16 [PMID: 32960481]
• Alba Blasco, Sílvia Gras, Guillem Mòdol‐Caballero, Olga Tarabal, Anna Casanovas, Lídia Piedrafita et al. Motoneuron deafferentation and gliosis occur in association with neuromuscular regressive changes during ageing in mice Journal of Cachexia, Sarcopenia and Muscle 2020-12-01 [PMID: 32691534]
• Bahar Shahidi, Michael C. Gibbons, Mary Esparza, Vinko Zlomislic, Richard Todd Allen, Steven R. Garfin et al. Cell populations and muscle fiber morphology associated with acute and chronic muscle degeneration in lumbar spine pathology JOR SPINE 2020-06-01 [PMID: 32613162]
• N Yamamoto, T Oyaizu, M Enomoto, M Horie, M Yuasa, A Okawa, K Yagishita VEGF and bFGF induction by nitric oxide is associated with hyperbaric oxygen-induced angiogenesis and muscle regeneration Sci Rep, 2020-02-17;10(1):2744. 2020-02-17 [PMID: 32066777] (IHC, Mouse)
• M Verma, Y Shimizu-Mo, Y Asakura, JP Ennen, J Bosco, Z Zhou, GH Fong, S Josiah, D Keefe, A Asakura Inhibition of FLT1 ameliorates muscular dystrophy phenotype by increased vasculature in a mouse model of Duchenne muscular dystrophy PLoS Genet., 2019-12-26;15(12):e1008468. 2019-12-26 [PMID: 31877123] (IHC frozen fixed, Mouse)
• Jin CL, Ye JL, Yang J mTORC1 Mediates Lysine-Induced Satellite Cell Activation to Promote Skeletal Muscle Growth Cells 11/30/2019 [PMID: 31801253]
• C Goossens, R Weckx, S Derde, T Dufour, S Vander Per, L Pauwels, SE Thiessen, PP Van Veldho, G Van den Be, L Langouche Adipose tissue protects against sepsis-induced muscle weakness in mice: from lipolysis to ketones Crit Care, 2019-07-01;23(1):236. 2019-07-01 [PMID: 31262340] (IHC-P, Mouse)
• L Melotti, E Vezzoli, F Mascarello, L Maccatrozz, M Patruno The natural involution of the sheep proximal sesamoidean ligament is due to depletion of satellite cells and simultaneous proliferation of fibroblasts: Ultrastructural evidence Res. Vet. Sci., 2019-03-08;124(0):106-111. 2019-03-08 [PMID: 30877991] (IHC-Fr, Ovine)
• J Wu, N Matthias, J Lo, JL Ortiz-Vita, AW Shieh, SH Wang, R Darabi A Myogenic Double-Reporter Human Pluripotent Stem Cell Line Allows Prospective Isolation of Skeletal Muscle Progenitors Cell Rep, 2018-11-13;25(7):1966-1981.e4. 11/13/2018 [PMID: 30428361]
• N Motohashi, A Uezumi, A Asakura, M Ikemoto-Ue, S Mori, Y Mizunoe, R Takashima, Y Miyagoe-Su, S Takeda, K Shigemoto Tbx1 regulates inherited metabolic and myogenic abilities of progenitor cells derived from slow- and fast-type muscle Cell Death Differ., 2018-08-28;0(0):. 2018-08-28 [PMID: 30154444] (IHC, Mouse)
• Y Kitajima, Y Ono Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line Skelet Muscle, 2018-08-24;8(1):26. 2018-08-24 [PMID: 30139390] (IHC-P, Mouse)
• CH Lim, JH Gil, H Quan, DH Viet, CK Kim Effect of 8-week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats Physiol Rep, 2018-06-01;6(12):e13725. 2018-06-01 [PMID: 29952091] (IHC, Rat)
• MS Huh, KG Young, K Yan, T Price-O'De, DJ Picketts Recovery from impaired muscle growth arises from prolonged postnatal accretion of myonuclei in Atrx mutant mice PLoS ONE, 2017-11-02;12(11):e0186989. 2017-11-02 [PMID: 29095838] (IHC-Fr, Mouse)
• Y Maeda, Y Yonemochi, Y Nakajyo, H Hidaka, T Ikeda, Y Ando CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration Sci Rep, 2017-06-12;7(1):3305. 2017-06-12 [PMID: 28607396] (IHC, Mouse)
• G Colaianni, T Mongelli, C Cuscito, P Pignataro, L Lippo, G Spiro, A Notarnicol, I Severi, G Passeri, G Mori, G Brunetti, B Moretti, U Tarantino, SC Colucci, JE Reseland, R Vettor, S Cinti, M Grano Irisin prevents and restores bone loss and muscle atrophy in hind-limb suspended mice Sci Rep, 2017-06-06;7(1):2811. 2017-06-06 [PMID: 28588307] (IHC, Mouse)
• B Shahidi, JC Hubbard, MC Gibbons, S Ruoss, V Zlomislic, RT Allen, SR Garfin, SR Ward Lumbar multifidus muscle degenerates in individuals with chronic degenerative lumbar spine pathology J. Orthop. Res., 2017-05-23;0(0):. 5/23/2017 [PMID: 28480978]
• A Shiraishi, K Muguruma, Y Sasai Generation of thalamic neurons from mouse embryonic stem cells Development, 2017-02-20;0(0):. 2017-02-20 [PMID: 28219951] (IHC, Mouse)
• BR Pryce, KN Al-Zahrani, S Dufresne, N Belkina, C Labr?che, G Patino-Lop, J Frenette, S Shaw, LA Sabourin Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness Skelet Muscle, 2017-02-02;7(1):3. 2017-02-02 [PMID: 28153048] (IHC, Mouse)
• Fuminori Kawano PRENATAL MYONUCLEI PLAY A CRUCIAL ROLE FOR SKELETAL MUSCLE HYPERTROPHY IN RODENTS Am. J. Physiol., Cell Physiol, 2016-12-07;0(0):ajpcell.00151. 2016-12-07 [PMID: 27927611] (IHC, Rat)
• Qilin Xu A Gingiva-Derived Mesenchymal Stem Cell-Laden Porcine Small Intestinal Submucosa Extracellular Matrix Construct Promotes Myomucosal Regeneration of the Tongue Tissue Eng Part A, 2017-01-04;0(0):. 2017-01-04 [PMID: 27923325] (IHC-P, Rat)
• Ratchakrit Srikuea, Muthita Hirunsai Effects of intramuscular administration of 1 alpha ,25(OH)2D3 during skeletal muscle regeneration on regenerative capacity, muscular fibrosis, and angiogenesis Journal of Applied Physiology 2016-06-15 [PMID: 27032903]
• James G. MacKrell, Benjamin C. Yaden, Heather Bullock, Keyue Chen, Pamela Shetler, Henry U. Bryant et al. Molecular Targets of Androgen Signaling that Characterize Skeletal Muscle Recovery and Regeneration Nuclear Receptor Signaling 2015-10-07 [PMID: 26457071]
• Theriault M, Pare M, Lemire B, Maltais F, Debigare R Regenerative defect in vastus lateralis muscle of patients with chronic obstructive pulmonary disease. Respir Res, 2014-03-25;15(0):35. 2014-03-25 [PMID: 24666540] (IHC, Human)
• Zachary S. Peacock, Brad J. Tricomi, Matthew E. Lawler, William C. Faquin, John C. Magill, Brian A. Murphy et al. Skeletal and Soft Tissue Response to Automated, Continuous, Curvilinear Distraction Osteogenesis Journal of Oral and Maxillofacial Surgery 2014-09-01 [PMID: 24602810] (Immunohistochemistry, Porcine)
• Brisson , Becky K, Spinazzola , Janelle, Park , SooHyun, Barton , Elisabet Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength. Am J Physiol Endocrinol Metab, 2014-02-25;306(8):E965-74. 2014-02-25 [PMID: 24569593] (IHC, Mouse)
• Riuzzi , Francesc, Sorci , Guglielm, Sagheddu , Roberta, Sidoni , Angelo, Alaggio , Rita, Ninfo , Vito, Donato , Rosario RAGE signaling deficiency in rhabdomyosarcoma cells causes upregulation of PAX7 and uncontrolled proliferation. J Cell Sci, 2014-02-19;127(0):1699-711. 2014-02-19 [PMID: 24554430] (IHC, Western Blot, Human)
• D'Arcy , Colleen, Feeney , Sandra J, McLean , Catriona, Gehrig , Stefan M, Lynch , Gordon S, Smith , Jaclyn E, Cowling , Belinda, Mitchell , Christin, McGrath , Meagan J Identification of FHL1 as a therapeutic target for Duchenne muscular dystrophy. Hum Mol Genet, 2013-09-18;23(3):618-36. 2013-09-18 [PMID: 24087791] (IHC, Mouse)
• Awaya , Tomonari, Kato , Takeo, Mizuno , Yuta, Chang , Hsi, Niwa , Akira, Umeda , Katsutsu, Nakahata , Tatsutos, Heike , Toshio Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells. PLoS ONE, 2012-12-07;7(12):e51638. 2012-12-07 [PMID: 23236522] (ICC, Human)
• A simplified but robust method for the isolation of avian and mammalian muscle satellite cells. BMC Cell Biol., 2012-06-21;13(0):16. 2012-06-21 [PMID: 22720831] (ICC, Avian - Chicken, Avian - Duck)
• Theriault ME, Pare MÈ, Maltais F, Debigare R Satellite cells senescence in limb muscle of severe patients with COPD. PLoS ONE, 2012-06-13;7(6):e39124. 2012-06-13 [PMID: 22720047] (IHC-Fr, Human)
• Rodrigues AM, Christen B, Marti M, Izpisua Belmonte JC Skeletal muscle regeneration in Xenopus tadpoles and zebrafish larvae. BMC Dev. Biol., 2012-02-27;12(0):9. 2012-02-27 [PMID: 22369050] (IHC-P, Xenopus, Zebrafish)
• Kallestad KM, Hebert SL, McDonald AA, Daniel ML, Cu SR, McLoon LK Sparing of extraocular muscle in aging and muscular dystrophies: a myogenic precursor cell hypothesis. Exp. Cell Res., 2011-01-27;317(6):873-85. 2011-01-27 [PMID: 21277300] (Flow Cytometry, Mouse)
• Morgan E. Carlson, Charlotte Suetta, Michael J. Conboy, Per Aagaard, Abigail Mackey, Michael Kjaer et al. Molecular aging and rejuvenation of human muscle stem cells EMBO Molecular Medicine 2010-01-06 [PMID: 20049743]
• Wilschut KJ, Haagsman HP, Roelen BA Extracellular matrix components direct porcine muscle stem cell behavior. Exp. Cell Res., 2009-10-22;316(3):341-52. 2009-10-22 [PMID: 19853598] (ICC, Porcine)
• Shi M, Ishikawa M, Kamei N, Nakasa T, Adachi N, Deie M, Asahara T, Ochi M Acceleration of skeletal muscle regeneration in a rat skeletal muscle injury model by local injection of human peripheral blood-derived CD133-positive cells. Stem Cells, 2009-04-01;27(4):949-60. 2009-04-01 [PMID: 19353523] (IHC, Rat)
• Ozasa S, Kimura S, Ito K, Ueno H, Ikezawa M, Matsukura M, Yoshioka K, Araki K, Yamamura KI, Abe K, Niwa H, Miike T Efficient conversion of ES cells into myogenic lineage using the gene-inducible system. Biochem. Biophys. Res. Commun., 2007-04-17;357(4):957-63. 2007-04-17 [PMID: 17466266] (ICC, Mouse)
• Zheng JK, Wang Y, Karandikar A, Wang Q, Gai H, Liu AL, Peng C, Sheng HZ Skeletal myogenesis by human embryonic stem cells. Cell Res., 2006-08-01;16(8):713-22. 2006-08-01 [PMID: 16788572] (ICC, Human)

Review for Pax7 Antibody (MAB1675) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Zachary Patinkin Simple Western Human 02/25/2015

Summary

• Application: Simple Western
• Sample Tested: Human skeletal muscle satellite-derived cell lysate
• Species: Human
• Lot: JKO0312101

Bioinformatics

• Gene Symbol: PAX7