Pax7 Antibody (PAX7) [Unconjugated]

Images

 
Pax7 was detected in immersion fixed C2C12 mouse myoblast cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) for 3 hours at room temperature. Cells were stained ...read more
Pax7 was detected in immersion fixed iBJ6 iPS cell line differentiated into myoblasts using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications ICC/IF
Clone
PAX7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Pax7 Antibody (PAX7) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant chicken Pax7
aa 352-523
Specificity
Detects human, mouse, rat, and chicken Pax7 (3-8).
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PAX7
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB1675 in the following application:

Publications
Read Publications using
MAB1675 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax7 Antibody (PAX7) [Unconjugated]

  • FLJ37460
  • HUP1
  • paired box 7
  • paired box gene 7
  • paired box homeotic gene 7
  • paired box protein Pax-7
  • paired domain gene 7
  • PAX7 transcriptional factor
  • Pax7
  • PAX7B
  • RMS2

Background

Pax7 belongs to the paired box protein family of transcription factors. It is an important regulator of neural and skeletal muscle development (1 - 3).
  1. Jostes, B. et al. (1990) Mech. Dev. 33(1):27.
  2. Asakura, A. et al. (2001) Differentiation 68(4-5):245.
  3. Kawakami, A. et al. (1997) Mech. Dev. 66:119.
  4. Ericson, J. et al. (1996) Cell 87:661.
  5. Berggren, K. et al. (2001) Dev. Dyn. 222:1.
  6. Takebayashi, H. et al. (2002) Mech. Dev. 113:169.
  7. McLoon, L.K. and J. Wirtschafter (2003) Invest. Opthamal. Vis. Sci. 44:1927.
  8. Yamamoto, S. et al. (2001) J. Neurosci. 21:9814.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pax7 Antibody (MAB1675)(26)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Avian - Chicken, Ovine, Porcine, Xenopus.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC frozen fixed, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(5)
IHC
(11)
IHC frozen fixed
(1)
IHC-Fr
(3)
IHC-P
(4)
Western Blot
(1)
All Applications
Filter By Species
Human
(5)
Mouse
(13)
Rat
(4)
Avian - Chicken
(1)
Ovine
(1)
Porcine
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using MAB1675 Applications Species
M Verma, Y Shimizu-Mo, Y Asakura, JP Ennen, J Bosco, Z Zhou, GH Fong, S Josiah, D Keefe, A Asakura Inhibition of FLT1 ameliorates muscular dystrophy phenotype by increased vasculature in a mouse model of Duchenne muscular dystrophy PLoS Genet., 2019;15(12):e1008468. 2019 [PMID: 31877123] (IHC frozen fixed, Mouse) IHC frozen fixed Mouse
C Goossens, R Weckx, S Derde, T Dufour, S Vander Per, L Pauwels, SE Thiessen, PP Van Veldho, G Van den Be, L Langouche Adipose tissue protects against sepsis-induced muscle weakness in mice: from lipolysis to ketones Crit Care, 2019;23(1):236. 2019 [PMID: 31262340] (IHC-P, Mouse) IHC-P Mouse
L Melotti, E Vezzoli, F Mascarello, L Maccatrozz, M Patruno The natural involution of the sheep proximal sesamoidean ligament is due to depletion of satellite cells and simultaneous proliferation of fibroblasts: Ultrastructural evidence Res. Vet. Sci., 2019;124(0):106-111. 2019 [PMID: 30877991] (IHC-Fr, Ovine) IHC-Fr Ovine
N Motohashi, A Uezumi, A Asakura, M Ikemoto-Ue, S Mori, Y Mizunoe, R Takashima, Y Miyagoe-Su, S Takeda, K Shigemoto Tbx1 regulates inherited metabolic and myogenic abilities of progenitor cells derived from slow- and fast-type muscle Cell Death Differ., 2018;0(0):. 2018 [PMID: 30154444] (IHC, Mouse) IHC Mouse
Y Kitajima, Y Ono Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line Skelet Muscle, 2018;8(1):26. 2018 [PMID: 30139390] (IHC-P, Mouse) IHC-P Mouse
CH Lim, JH Gil, H Quan, DH Viet, CK Kim Effect of 8-week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats Physiol Rep, 2018;6(12):e13725. 2018 [PMID: 29952091] (IHC, Rat) IHC Rat
MS Huh, KG Young, K Yan, T Price-O'De, DJ Picketts Recovery from impaired muscle growth arises from prolonged postnatal accretion of myonuclei in Atrx mutant mice PLoS ONE, 2017;12(11):e0186989. 2017 [PMID: 29095838] (IHC-Fr, Mouse) IHC-Fr Mouse
Y Maeda, Y Yonemochi, Y Nakajyo, H Hidaka, T Ikeda, Y Ando CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration Sci Rep, 2017;7(1):3305. 2017 [PMID: 28607396] (IHC, Mouse) IHC Mouse
G Colaianni, T Mongelli, C Cuscito, P Pignataro, L Lippo, G Spiro, A Notarnicol, I Severi, G Passeri, G Mori, G Brunetti, B Moretti, U Tarantino, SC Colucci, JE Reseland, R Vettor, S Cinti, M Grano Irisin prevents and restores bone loss and muscle atrophy in hind-limb suspended mice Sci Rep, 2017;7(1):2811. 2017 [PMID: 28588307] (IHC, Mouse) IHC Mouse
A Shiraishi, K Muguruma, Y Sasai Generation of thalamic neurons from mouse embryonic stem cells Development, 2017;0(0):. 2017 [PMID: 28219951] (IHC, Mouse) IHC Mouse
Show All 26 Publications.

Review for Pax7 Antibody (MAB1675) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB1675:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western Pax7 MAB1675
Enlarge
4
reviewed by:
Zachary Patinkin
Simple Western Human 02/25/2015
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Summary

ApplicationSimple Western
Sample TestedHuman skeletal muscle satellite-derived cell lysate
SpeciesHuman
LotJKO0312101

Blocking

Blocking DetailsProtein Simple Antibody Diluent, 5 min, 25C

Primary Anitbody

Dilution Ratio10 ug/mL, 30 min, Protein Simple Antibody Diluent

Secondary Antibody

Secondary DescriptionProtein Simple Anti-Rabbit Secondary
Secondary Manufacturer Cat#042-206
Secondary Concentrationundiluted

Details

Detection NotesWES multi-image analysis

Comments

CommentsSimple Western.

FAQs for Pax7 Antibody (MAB1675) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Zachary Patinkin
02/25/2015
Application: Simple Western
Species: Human

Bioinformatics

Gene Symbol PAX7