Pax7 Antibody (PAX7) [Unconjugated]

Pax7 was detected in immersion fixed C2C12 mouse myoblast cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) for 3 hours at room temperature. Cells were stained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Pax7 was detected in immersion fixed iBJ6 iPS cell line differentiated into myoblasts using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: ICC/IF
• Clone: PAX7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Pax7 Antibody (PAX7) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant chicken Pax7
  aa 352-523
• Specificity: Detects human, mouse, rat, and chicken Pax7 (3-8).
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAX7

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax7 Antibody (PAX7) [Unconjugated]

• FLJ37460
• HUP1
• paired box 7
• paired box gene 7
• paired box homeotic gene 7
• paired box protein Pax-7
• paired domain gene 7
• PAX7 transcriptional factor
• Pax7
• PAX7B
• RMS2

Background

Pax7 belongs to the paired box protein family of transcription factors. It is an important regulator of neural and skeletal muscle development (1 - 3).

1. Jostes, B. et al. (1990) Mech. Dev. 33(1):27.
2. Asakura, A. et al. (2001) Differentiation 68(4-5):245.
3. Kawakami, A. et al. (1997) Mech. Dev. 66:119.
4. Ericson, J. et al. (1996) Cell 87:661.
5. Berggren, K. et al. (2001) Dev. Dyn. 222:1.
6. Takebayashi, H. et al. (2002) Mech. Dev. 113:169.
7. McLoon, L.K. and J. Wirtschafter (2003) Invest. Opthamal. Vis. Sci. 44:1927.
8. Yamamoto, S. et al. (2001) J. Neurosci. 21:9814.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pax7 Antibody (MAB1675)(26)

Publications using MAB1675

• M Verma, Y Shimizu-Mo, Y Asakura, JP Ennen, J Bosco, Z Zhou, GH Fong, S Josiah, D Keefe, A Asakura Inhibition of FLT1 ameliorates muscular dystrophy phenotype by increased vasculature in a mouse model of Duchenne muscular dystrophy PLoS Genet., 2019;15(12):e1008468. 2019 [PMID: 31877123] (IHC frozen fixed, Mouse)
• C Goossens, R Weckx, S Derde, T Dufour, S Vander Per, L Pauwels, SE Thiessen, PP Van Veldho, G Van den Be, L Langouche Adipose tissue protects against sepsis-induced muscle weakness in mice: from lipolysis to ketones Crit Care, 2019;23(1):236. 2019 [PMID: 31262340] (IHC-P, Mouse)
• L Melotti, E Vezzoli, F Mascarello, L Maccatrozz, M Patruno The natural involution of the sheep proximal sesamoidean ligament is due to depletion of satellite cells and simultaneous proliferation of fibroblasts: Ultrastructural evidence Res. Vet. Sci., 2019;124(0):106-111. 2019 [PMID: 30877991] (IHC-Fr, Ovine)
• N Motohashi, A Uezumi, A Asakura, M Ikemoto-Ue, S Mori, Y Mizunoe, R Takashima, Y Miyagoe-Su, S Takeda, K Shigemoto Tbx1 regulates inherited metabolic and myogenic abilities of progenitor cells derived from slow- and fast-type muscle Cell Death Differ., 2018;0(0):. 2018 [PMID: 30154444] (IHC, Mouse)
• Y Kitajima, Y Ono Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line Skelet Muscle, 2018;8(1):26. 2018 [PMID: 30139390] (IHC-P, Mouse)
• CH Lim, JH Gil, H Quan, DH Viet, CK Kim Effect of 8-week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats Physiol Rep, 2018;6(12):e13725. 2018 [PMID: 29952091] (IHC, Rat)
• MS Huh, KG Young, K Yan, T Price-O'De, DJ Picketts Recovery from impaired muscle growth arises from prolonged postnatal accretion of myonuclei in Atrx mutant mice PLoS ONE, 2017;12(11):e0186989. 2017 [PMID: 29095838] (IHC-Fr, Mouse)
• Y Maeda, Y Yonemochi, Y Nakajyo, H Hidaka, T Ikeda, Y Ando CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration Sci Rep, 2017;7(1):3305. 2017 [PMID: 28607396] (IHC, Mouse)
• G Colaianni, T Mongelli, C Cuscito, P Pignataro, L Lippo, G Spiro, A Notarnicol, I Severi, G Passeri, G Mori, G Brunetti, B Moretti, U Tarantino, SC Colucci, JE Reseland, R Vettor, S Cinti, M Grano Irisin prevents and restores bone loss and muscle atrophy in hind-limb suspended mice Sci Rep, 2017;7(1):2811. 2017 [PMID: 28588307] (IHC, Mouse)
• A Shiraishi, K Muguruma, Y Sasai Generation of thalamic neurons from mouse embryonic stem cells Development, 2017;0(0):. 2017 [PMID: 28219951] (IHC, Mouse)
• BR Pryce, KN Al-Zahrani, S Dufresne, N Belkina, C Labr?che, G Patino-Lop, J Frenette, S Shaw, LA Sabourin Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness Skelet Muscle, 2017;7(1):3. 2017 [PMID: 28153048] (IHC, Mouse)
• Fuminori Kawano PRENATAL MYONUCLEI PLAY A CRUCIAL ROLE FOR SKELETAL MUSCLE HYPERTROPHY IN RODENTS Am. J. Physiol., Cell Physiol, 2016;0(0):ajpcell.00151. 2016 [PMID: 27927611] (IHC, Rat)
• Qilin Xu A Gingiva-Derived Mesenchymal Stem Cell-Laden Porcine Small Intestinal Submucosa Extracellular Matrix Construct Promotes Myomucosal Regeneration of the Tongue Tissue Eng Part A, 2016;0(0):. 2016 [PMID: 27923325] (IHC-P, Rat)
• Theriault M, Pare M, Lemire B, Maltais F, Debigare R Regenerative defect in vastus lateralis muscle of patients with chronic obstructive pulmonary disease. Respir Res, 2014;15(0):35. 2014 [PMID: 24666540] (IHC, Human)
• Brisson, Becky K, Spinazzola, Janelle, Park, SooHyun, Barton, Elisabet Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength. Am J Physiol Endocrinol Metab, 2014;306(8):E965-74. 2014 [PMID: 24569593] (IHC, Mouse)
• Riuzzi, Francesc, Sorci, Guglielm, Sagheddu, Roberta, Sidoni, Angelo, Alaggio, Rita, Ninfo, Vito, Donato, Rosario RAGE signaling deficiency in rhabdomyosarcoma cells causes upregulation of PAX7 and uncontrolled proliferation. J Cell Sci, 2014;127(0):1699-711. 2014 [PMID: 24554430] (Western Blot, Human)
• D'Arcy, Colleen, Feeney, Sandra J, McLean, Catriona, Gehrig, Stefan M, Lynch, Gordon S, Smith, Jaclyn E, Cowling, Belinda, Mitchell, Christin, McGrath, Meagan J Identification of FHL1 as a therapeutic target for Duchenne muscular dystrophy. Hum Mol Genet, 2014;23(3):618-36. 2014 [PMID: 24087791] (IHC, Mouse)
• Awaya, Tomonari, Kato, Takeo, Mizuno, Yuta, Chang, Hsi, Niwa, Akira, Umeda, Katsutsu, Nakahata, Tatsutos, Heike, Toshio Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells. PLoS ONE, 2012;7(12):e51638. 2012 [PMID: 23236522] (ICC, Human)
• A simplified but robust method for the isolation of avian and mammalian muscle satellite cells. BMC Cell Biol., 2012;13(0):16. 2012 [PMID: 22720831] (ICC, Avian - Chicken)
• Theriault ME, Pare MÈ, Maltais F, Debigare R Satellite cells senescence in limb muscle of severe patients with COPD. PLoS ONE, 2012;7(6):e39124. 2012 [PMID: 22720047] (IHC-Fr, Human)
• Rodrigues AM, Christen B, Marti M, Izpisua Belmonte JC Skeletal muscle regeneration in Xenopus tadpoles and zebrafish larvae. BMC Dev. Biol., 2012;12(0):9. 2012 [PMID: 22369050] (IHC-P, Xenopus)
• Kallestad KM, Hebert SL, McDonald AA, Daniel ML, Cu SR, McLoon LK Sparing of extraocular muscle in aging and muscular dystrophies: a myogenic precursor cell hypothesis. Exp. Cell Res., 2011;317(6):873-85. 2011 [PMID: 21277300] (Flow Cytometry, Mouse)
• Wilschut KJ, Haagsman HP, Roelen BA Extracellular matrix components direct porcine muscle stem cell behavior. Exp. Cell Res., 2010;316(3):341-52. 2010 [PMID: 19853598] (ICC, Porcine)
• Shi M, Ishikawa M, Kamei N, Nakasa T, Adachi N, Deie M, Asahara T, Ochi M Acceleration of skeletal muscle regeneration in a rat skeletal muscle injury model by local injection of human peripheral blood-derived CD133-positive cells. Stem Cells, 2009;27(4):949-60. 2009 [PMID: 19353523] (IHC, Rat)
• Ozasa S, Kimura S, Ito K, Ueno H, Ikezawa M, Matsukura M, Yoshioka K, Araki K, Yamamura KI, Abe K, Niwa H, Miike T Efficient conversion of ES cells into myogenic lineage using the gene-inducible system. Biochem. Biophys. Res. Commun., 2007;357(4):957-63. 2007 [PMID: 17466266] (ICC, Mouse)
• Zheng JK, Wang Y, Karandikar A, Wang Q, Gai H, Liu AL, Peng C, Sheng HZ Skeletal myogenesis by human embryonic stem cells. Cell Res., 2006;16(8):713-22. 2006 [PMID: 16788572] (ICC, Human)

Review for Pax7 Antibody (MAB1675) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Zachary Patinkin Simple Western Human 02/25/2015

Summary

• Application: Simple Western
• Sample Tested: Human skeletal muscle satellite-derived cell lysate
• Species: Human
• Lot: JKO0312101

Blocking

• Blocking Details: Protein Simple Antibody Diluent, 5 min, 25C

Primary Anitbody

• Dilution Ratio: 10 ug/mL, 30 min, Protein Simple Antibody Diluent

Secondary Antibody

• Secondary Description: Protein Simple Anti-Rabbit Secondary
• Secondary Manufacturer Cat#: 042-206
• Secondary Concentration: undiluted

Details

• Detection Notes: WES multi-image analysis

Comments

• Comments: Simple Western.

Bioinformatics

• Gene Symbol: PAX7