Pax7 Antibody (PAX7) [Unconjugated]

Images

 
Pax7 was detected in immersion fixed C2C12 mouse myoblast cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) for 3 hours at room temperature. Cells were stained ...read more
Pax7 was detected in immersion fixed iBJ6 iPS cell line differentiated into myoblasts using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 10 µg/mL for 3 hours at room temperature. ...read more
Pax7 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 5 µg/mL for 1 hour at room ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications IHC, ICC/IF
Clone
PAX7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Pax7 Antibody (PAX7) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant chicken Pax7
aa 352-523
Specificity
Detects human, mouse, rat, and chicken Pax7 (3-8).
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PAX7
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB1675 in the following applications:

Publications
Read Publications using
MAB1675 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax7 Antibody (PAX7) [Unconjugated]

  • FLJ37460
  • HUP1
  • paired box 7
  • paired box gene 7
  • paired box homeotic gene 7
  • paired box protein Pax-7
  • paired domain gene 7
  • PAX7 transcriptional factor
  • Pax7
  • PAX7B
  • RMS2

Background

Pax7 belongs to the paired box protein family of transcription factors. It is an important regulator of neural and skeletal muscle development (1 - 3).
  1. Jostes, B. et al. (1990) Mech. Dev. 33(1):27.
  2. Asakura, A. et al. (2001) Differentiation 68(4-5):245.
  3. Kawakami, A. et al. (1997) Mech. Dev. 66:119.
  4. Ericson, J. et al. (1996) Cell 87:661.
  5. Berggren, K. et al. (2001) Dev. Dyn. 222:1.
  6. Takebayashi, H. et al. (2002) Mech. Dev. 113:169.
  7. McLoon, L.K. and J. Wirtschafter (2003) Invest. Opthamal. Vis. Sci. 44:1927.
  8. Yamamoto, S. et al. (2001) J. Neurosci. 21:9814.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pax7 Antibody (MAB1675)(48)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Avian - Chicken, Avian - Duck, Ovine, Porcine, Xenopus, Zebrafish.

We have publications tested in 9 applications: Flow Cytometry, ICC, ICC/IF, IHC, IHC frozen fixed, IHC-Fr, IHC-P, IHC/IF, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(7)
ICC/IF
(1)
IHC
(14)
IHC frozen fixed
(1)
IHC-Fr
(3)
IHC-P
(4)
IHC/IF
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(17)
Rat
(5)
Avian - Chicken
(1)
Avian - Duck
(1)
Ovine
(1)
Porcine
(1)
Xenopus
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 48. Show All 48 Publications.
Publications using MAB1675 Applications Species
Lim, C;McKendry, J;Giacomin, T;Mcleod, JC;Ng, SY;Currier, BS;Coletta, G;Phillips, SM; Fortetropin supplementation prevents the rise in circulating myostatin but not disuse-induced muscle atrophy in young men with limb immobilization: A randomized controlled trial PloS one 2023-05-23 [PMID: 37220119] (IHC, Human) IHC Human
Y Cao, J Tan, H Zhao, T Deng, Y Hu, J Zeng, J Li, Y Cheng, J Tang, Z Hu, K Hu, B Xu, Z Wang, Y Wu, PE Lobie, S Ma Bead-jet printing enabled sparse mesenchymal stem cell patterning augments skeletal muscle and hair follicle regeneration Nature Communications, 2022-12-03;13(1):7463. 2022-12-03 [PMID: 36460667] (IHC/IF, Mouse) IHC/IF Mouse
T Zhang, C Künne, D Ding, S Günther, X Guo, Y Zhou, X Yuan, T Braun Replication collisions induced by de-repressed S-phase transcription are connected with malignant transformation of adult stem cells Nature Communications, 2022-11-14;13(1):6907. 2022-11-14 [PMID: 36376321] (ICC, Mouse) ICC Mouse
Yamakawa D, Tsuboi J, Kasahara K et al. Cilia-Mediated Insulin/Akt and ST2/JNK Signaling Pathways Regulate the Recovery of Muscle Injury Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11/14/2022 [PMID: 36373718]

Details:
Dilution used in WB 1:1000
David W. Hammers NOX4 inhibition promotes the remodeling of dystrophic muscle JCI Insight 2022-10-24 [PMID: 36278481]
I Kim, A Ghosh, N Bundschuh, L Hinte, E Petrosyan, F von Meyenn, O Bar-Nur Integrative molecular roadmap for direct conversion of fibroblasts into myocytes and myogenic progenitor cells Science Advances, 2022-04-06;8(14):eabj4928. 2022-04-06 [PMID: 35385316] (ICC/IF, Mouse) ICC/IF Mouse
Sílvia Gras, Alba Blasco, Guillem Mòdol-Caballero, Olga Tarabal, Anna Casanovas, Lídia Piedrafita et al. Beneficial effects of dietary supplementation with green tea catechins and cocoa flavanols on aging-related regressive changes in the mouse neuromuscular system Aging (Albany NY) 2021-07-31 [PMID: 34319911]
C Gong, X Zheng, F Guo, Y Wang, S Zhang, J Chen, X Sun, SZA Shah, Y Zheng, X Li, Y Yin, Q Li, X Huang, T Guo, X Han, SC Zhang, W Wang, H Chen Human spinal GABA neurons alleviate spasticity and improve locomotion in rats with spinal cord injury Cell Reports, 2021-03-23;34(12):108889. 2021-03-23 [PMID: 33761348] (ICC, Rat) ICC Rat
Mario Parstorfer, Francesca Profit, Nadine Weiberg, Michaela Wehrstein, Alexander Barié, Birgit Friedmann-Bette Increased satellite cell apoptosis in vastus lateralis muscle after anterior cruciate ligament reconstruction Journal of Rehabilitation Medicine 2021-02-17 [PMID: 33569607]
Meng-Huang Wu, Chuang-Yu Lin, Chun-Yin Hou, Ming-Thau Sheu, Hsi Chang Micronized sacchachitin promotes satellite cell proliferation through TAK1-JNK-AP-1 signaling pathway predominantly by TLR2 activation Chinese Medicine 2020-12-01 [PMID: 33514380]
Show All 48 Publications.

Review for Pax7 Antibody (MAB1675) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB1675:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western Pax7 MAB1675
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4
reviewed by:
Zachary Patinkin
Simple Western Human 02/25/2015
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Summary

ApplicationSimple Western
Sample TestedHuman skeletal muscle satellite-derived cell lysate
SpeciesHuman
LotJKO0312101

FAQs for Pax7 Antibody (MAB1675) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

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Recent Reviews

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Zachary Patinkin
02/25/2015
Application: Simple Western
Species: Human

Bioinformatics

Gene Symbol PAX7