MYH6 Antibody (3-48) - Azide and BSA Free

Western Blot: MYH6 Antibody (3-48) [NB300-284] - analysis of total cardiac myosin expression in tissue lysates of rat heart (lanes 1-5), liver (lanes 6-7), and lung (lanes 8-9). Increasing amounts of total protein were loaded as follow: lanes 1, 6 and 8: 0 ug; lane 2: 50 ug; lanes 3 , 7 and 9: 250 ug; lane 5: 500 ug. (anti-cMHC was used at 1:500; secondary antibody: Goat anti-mouse IgG Fc-HRP; 1:5000)

Immunohistochemistry-Paraffin: MYH6 Antibody (3-48) [NB300-284] - Formaldehyde-fixed paraffin-embedded (FFPE) Tissue Slides. Human heart ventricle following immunostaining with cMHC 3-48 and irrelevant NeuAc monoclonal antibody at low microscopical magnification.

Western Blot: MYH6 Antibody (3-48) [NB300-284] - Western blot immunodetection of cardiac MyHC isoforms from left ventricle (LV) homogenates and extracts of adult euthyroid (EU), hypothyroid (HY), and hyperthyroid (TH) Lewis rats. The membranes were stained by NB300-284 antibody recognizing both MyHCalpha and MyHCbeta isoforms. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2011/634253/), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, Ca, Bv, Rb
• Applications: WB, ICC/IF, IHC, IHC-P
• Clone: 3-48
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

MYH6 Antibody (3-48) - Azide and BSA Free Summary

• Immunogen: Human ventricular myosin
• Specificity: This antibody recognizes alpha and beta type heavy chains of light meromyosin fragments (LMM) of cardiac myosin heavy chains. It also reacts with LMM fragments of cardiac myosin and beta heavy chain of slow human skeletal muscle. The antibody does not crossreact with human myosin light chains.
• Predicted Species: Bovine (97%), Rabbit (91%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYH6
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:300
  • Immunohistochemistry-Paraffin 1:300
  • Western Blot 1:500
• Application Notes: Please only use IgG speciific secondaries with this product. Fab region specific secondaries may not detect this antibody. ICC/IF reactivity reported in (PMID: 23577316).

Reactivity Notes

Canine reactivity reported in (PMID: 23577316). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Predicted to react with Rabbit, Bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized protein G purified in PBS pH7.4 (Full IgG)
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for MYH6 Antibody (3-48) - Azide and BSA Free

• alpha-MHC
• ASD3
• cardiomyopathy
• CMD1EE
• CMH14
• Hypertrophic 1
• MYH6
• MYHC
• MYHCA
• MyHC-Alpha
• Myosin Heavy Chain 6
• Myosin Heavy Chain, Cardiac Muscle Alpha Isoform
• Myosin, Heavy Chain 6, Cardiac Muscle, Alpha
• Myosin, Heavy Polypeptide 6, Cardiac Muscle, Alpha (Cardiomyopathy, Hypertrophic 1)
• Myosin-6
• SSS3

Background

Myosin is a highly conserved, ubiquitously expressed protein that hydrolyzes ATP, and this reaction provides the energy required for muscle contraction and that interacts with Actin to generate the force for cellular movements. It is a hexameric protein composed of four light chains and two heavy chains, each containing an actin-binding site and an ATP hydrolysis site. The heavy chains are encoded by the MYH gene family and were first isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Cardiac myosin exists as two isoforms in humans, a-cardiac myosins and b-cardiac myosins. These two isoforms are expressed in different amounts in the human heart. b-cardiac myosins is the predominant form during normal physiology while the a-isoform contributes for approximately 7% of the total myosin. Mutations of the MYH genes are associated with several different dilated and hypertrophic cardiomyopathies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MYH6 Antibody (NB300-284)(10)

Publications using NB300-284

• Nonaka K, Akiyama J, Yoshikawa Y et al. 1,25-Dihydroxyvitamin D3 Inhibits Lipopolysaccharide-Induced Interleukin-6 Production by C2C12 Myotubes Medicina (Kaunas) 2020-09-04 [PMID: 32899782] (WB, Mouse)
• Berkholz J, Kuzyniak W, Hoepfner M, Munz B. Overexpression of the skNAC gene in human rhabdomyosarcoma cells enhances their differentiation potential and inhibits tumor cell growth and spreading. Clin Exp Metastasis. 2014-09-11 [PMID: 25209525]
• Berkholz J, Michalick L, Munz B. The E3 SUMO ligase Nse2 regulates sumoylation and nuclear-to-cytoplasmic translocation of skNAC-Smyd1 in myogenesis. J. Cell. Sci. 2014-07-07 [PMID: 25002400] (WB, Mouse)
  
  Details:
  Myosin heavy chain antibody (clone 3-48) used in WB application as myogenic differentiation markers on C2C12 cells, a mouse myoblast cell line, transfected or not with Nse2-specific siRNA - siNse2 (Figure 3C).
• Suzuki K, Kishiok Y, Wakamatsu J-I, Nishimura T. Decorin activates Akt downstream of IGF-IR and promotes myoblast differentiation Animal Science Journal 2013-03-27 [PMID: 23607268] (WB, Mouse)
• Kang SB, Lee HS, Lim JY et al. Injection of porous polycaprolactone beads containing autologous myoblasts in a dog model of fecal incontinence J Korean Surg Soc 2013-04-01 [PMID: 23577316] (ICC/IF, IHC-P, Canine)
• Broderick TL, Jankowski M, Wang D et al. Downregulation in GATA4 and Downstream Structural and Contractile Genes in the db/db Mouse Heart ISRN Endocrinology, vol 2012-01-01 [PMID: 22474596] (WB, Mouse)
• Arnostova P, Jedelsky PL, Soukup T, Zurmanova J. Electrophoretic mobility of cardiac myosin heavy chain isoforms revisited: application of MALDI TOF/TOF analysis. J Biomed Biotechnol 2011-01-01 [PMID: 22187528] (Rat)
• Berger F, Berkholz J, Breustedt T et al. Skeletal muscle-specific variant of nascent polypeptide associated complex alpha (skNAC): Implications for a specific role in mammalian myoblast differentiation. European Journal of Cell Biology. 2011-12-09 [PMID: 22154550]
• Ott HC, Matthiesen TS, Goh SK et al. Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart. Nat Med 2008-02-01 [PMID: 18193059] (Rat)
• Barrett PL Nrg-1 effects on C2C12 differentiation: Towards generating Intrafusal muscle fibres in vitro Thesis 2022-01-01

Reviews for MYH6 Antibody (NB300-284) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Rat.

reviewed by: Hong Li WB Human 11/25/2014

Summary

• Application: Western Blot
• Species: Human

reviewed by: Yi-Fan Lion WB Rat 12/30/2011

Summary

• Application: Western Blot
• Sample Tested: Rat
• Species: Rat

FAQs for MYH6 Antibody (NB300-284). (Showing 1 - 1 of 1 FAQs).

1. We are looking for a Myosin heavy chain antibody. The sample is from mouse, and I would like to perform ICC. I found NB300-284. From its specific reference (http://www.sciencedirect.com/science/article/pii/S0171933511002123), there are ICC images. However, I cannot confirm if those ICC images are produced by NB300-284. Is it possible for you to confirm for us?
   • The catalog # NB300-284 refers to our myosin heavy chain antibody (3-48) and this antibody is generated from clone 3-48. If you see page number 151 of the publication (Eur J Cell Biol. 2012 Feb;91(2):150-5. Epub 2011 Dec 10), you can see a mention on Novus's myosin heavy chain antibody with clone 3-48 that was used for ICC-IF and WB applications.

Bioinformatics

• Gene Symbol: MYH6
• Entrez:
  • 79784(Human)
  • 71960(Mouse)
  • 308572(Rat)
• Uniprot:
  • Q7Z406(Human)