MYH6 Antibody (3-48)

Images

 
Western Blot: MYH6 Antibody (3-48) [NB300-284] - analysis of total cardiac myosin expression in tissue lysates of rat heart (lanes 1-5), liver (lanes 6-7), and lung (lanes 8-9). Increasing amounts of total protein were ...read more
Immunohistochemistry-Paraffin: MYH6 Antibody (3-48) [NB300-284] - Formaldehyde-fixed paraffin-embedded (FFPE) Tissue Slides. Human heart ventricle following immunostaining with cMHC 3-48 and irrelevant NeuAc monoclonal ...read more
Western Blot: MYH6 Antibody (3-48) [NB300-284] - Western blot immunodetection of cardiac MyHC isoforms from left ventricle (LV) homogenates and extracts of adult euthyroid (EU), hypothyroid (HY), and hyperthyroid (TH) ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Bv, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
3-48
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

MYH6 Antibody (3-48) Summary

Immunogen
Human ventricular myosin
Specificity
This antibody recognizes alpha and beta type heavy chains of light meromyosin fragments (LMM) of cardiac myosin heavy chains. It also reacts with LMM fragments of cardiac myosin and beta heavy chain of slow human skeletal muscle. The antibody does not crossreact with human myosin light chains.
Predicted Species
Bovine (97%), Rabbit (91%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYH6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:300
  • Immunohistochemistry-Paraffin 1:300
Application Notes
Please only use IgG speciific secondaries with this product. Fab region specific secondaries may not detect this antibody. ICC/IF reactivity reported in (PMID: 23577316).
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB300-284 in the following applications:

Publications
Read Publications using
NB300-284 in the following applications:

Reactivity Notes

Canine reactivity reported in (PMID: 23577316). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Predicted to react with Rabbit, Bovine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS pH7.4
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified
Reconstitution Instructions
Reconstitute in 100 ul of distilled H2O for a 1 ug/ul solution.

Alternate Names for MYH6 Antibody (3-48)

  • alpha-MHC
  • ASD3
  • cardiomyopathy
  • CMD1EE
  • CMH14
  • Hypertrophic 1
  • MYH6
  • MYHC
  • MYHCA
  • MyHC-Alpha
  • Myosin Heavy Chain 6
  • Myosin Heavy Chain, Cardiac Muscle Alpha Isoform
  • Myosin, Heavy Chain 6, Cardiac Muscle, Alpha
  • Myosin, Heavy Polypeptide 6, Cardiac Muscle, Alpha (Cardiomyopathy, Hypertrophic 1)
  • Myosin-6
  • SSS3

Background

Myosin is a highly conserved, ubiquitously expressed protein that hydrolyzes ATP, and this reaction provides the energy required for muscle contraction and that interacts with Actin to generate the force for cellular movements. It is a hexameric protein composed of four light chains and two heavy chains, each containing an actin-binding site and an ATP hydrolysis site. The heavy chains are encoded by the MYH gene family and were first isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Cardiac myosin exists as two isoforms in humans, a-cardiac myosins and b-cardiac myosins. These two isoforms are expressed in different amounts in the human heart. b-cardiac myosins is the predominant form during normal physiology while the a-isoform contributes for approximately 7% of the total myosin. Mutations of the MYH genes are associated with several different dilated and hypertrophic cardiomyopathies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-284
Species: Hu, Mu, Rt, Ca, Bv, Rb
Applications: WB, ICC/IF, IHC, IHC-P

Publications for MYH6 Antibody (NB300-284)(9)

We have publications tested in 3 confirmed species: Mouse, Rat, Canine.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
WB
(4)
All Applications
Filter By Species
Mouse
(4)
Rat
(2)
Canine
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB300-284 Applications Species
Nonaka K, Akiyama J, Yoshikawa Y et al. 1,25-Dihydroxyvitamin D3 Inhibits Lipopolysaccharide-Induced Interleukin-6 Production by C2C12 Myotubes Medicina (Kaunas) Sep 4 2020 [PMID: 32899782] (WB, Mouse) WB Mouse
Berkholz J, Kuzyniak W, Hoepfner M, Munz B. Overexpression of the skNAC gene in human rhabdomyosarcoma cells enhances their differentiation potential and inhibits tumor cell growth and spreading. Clin Exp Metastasis. 2014 Sep 11 [PMID: 25209525]
Berkholz J, Michalick L, Munz B. The E3 SUMO ligase Nse2 regulates sumoylation and nuclear-to-cytoplasmic translocation of skNAC-Smyd1 in myogenesis. J. Cell. Sci. 2014 Jul 07 [PMID: 25002400] (WB, Mouse)

Details:
Myosin heavy chain antibody (clone 3-48) used in WB application as myogenic differentiation markers on C2C12 cells, a mouse myoblast cell line, transfected or not with Nse2-specific siRNA - siNse2 (Figure 3C).
WB Mouse
Suzuki K, Kishiok Y, Wakamatsu J-I, Nishimura T. Decorin activates Akt downstream of IGF-IR and promotes myoblast differentiation Animal Science Journal 2013 Mar 27 [PMID: 23607268] (WB, Mouse) WB Mouse
Kang SB, Lee HS, Lim JY et al. Injection of porous polycaprolactone beads containing autologous myoblasts in a dog model of fecal incontinence J Korean Surg Soc 2013 Apr [PMID: 23577316] (ICC/IF, IHC-P, Canine) ICC/IF, IHC-P Canine
Broderick TL, Jankowski M, Wang D et al. Downregulation in GATA4 and Downstream Structural and Contractile Genes in the db/db Mouse Heart ISRN Endocrinology, vol 2012 [PMID: 22474596] (WB, Mouse) WB Mouse
Arnostova P, Jedelsky PL, Soukup T, Zurmanova J. Electrophoretic mobility of cardiac myosin heavy chain isoforms revisited: application of MALDI TOF/TOF analysis. J Biomed Biotechnol 2011 [PMID: 22187528] (Rat) Rat
Berger F, Berkholz J, Breustedt T et al. Skeletal muscle-specific variant of nascent polypeptide associated complex alpha (skNAC): Implications for a specific role in mammalian myoblast differentiation. European Journal of Cell Biology. 2011 Dec 9. [PMID: 22154550]
Ott HC, Matthiesen TS, Goh SK et al. Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart. Nat Med 2008 Feb [PMID: 18193059] (Rat) Rat

Reviews for MYH6 Antibody (NB300-284) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using NB300-284:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MYH6 NB300-284
Enlarge
5
reviewed by:
Anonymous
WB Human 11/25/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF
  4
reviewed by:
Anonymous
WB Rat 12/30/2011
View

Summary

ApplicationWestern Blot
Sample TestedRat
SpeciesRat

Blocking

Blocking DetailsBlocking Buffer: BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse HRP

Details

Detection NotesDetection Method: ECL

Product General Protocols

View specific protocols for MYH6 Antibody (NB300-284): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MYH6 Antibody (NB300-284). (Showing 1 - 1 of 1 FAQs).

  1. We are looking for a Myosin heavy chain antibody. The sample is from mouse, and I would like to perform ICC. I found NB300-284. From its specific reference (http://www.sciencedirect.com/science/article/pii/S0171933511002123), there are ICC images. However, I cannot confirm if those ICC images are produced by NB300-284. Is it possible for you to confirm for us?
    • The catalog # NB300-284 refers to our myosin heavy chain antibody (3-48) and this antibody is generated from clone 3-48. If you see page number 151 of the publication (Eur J Cell Biol. 2012 Feb;91(2):150-5. Epub 2011 Dec 10), you can see a mention on Novus's myosin heavy chain antibody with clone 3-48 that was used for ICC-IF and WB applications.

Secondary Antibodies

 

Isotype Controls

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Array NB300-284

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
11/25/2014
Application: WB
Species: Human

 
Anonymous
12/30/2011
Application: WB
Species: Rat

Bioinformatics

Gene Symbol MYH6
Entrez
Uniprot