Myeloperoxidase/MPO Antibody

Product Discontinued

Myeloperoxidase/MPO Antibody Summary

• Immunogen: Myeloperoxidase isolated from human polymorphonuclear leucocytes
• Specificity: This antiserum reacts with human myeloperoxidase.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MPO
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: IHC, WB. This antiserum works superbly for staining of paraffin-embedded tissue sections fixed in formalin, frozen sections and cell cytospins. The optimal dilution should be determined by the end user.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute with deionized water.

Alternate Names for Myeloperoxidase/MPO Antibody

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms.CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O.CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O.COFACTOR: Binds 1 calcium ion per heterodimer.COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer.SUBUNIT: Tetramer of two light chains and two heavy chains.SUBCELLULAR LOCATION: Lysosome.ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing.DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis.SIMILARITY: Belongs to the peroxidase family. XPO subfamily.



This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myeloperoxidase/MPO Antibody (R-133-250)(5)

Publications using R-133-250

• Arber DA, Snyder DS, Fine M, Dagis A, NilJ, Slovak ML. Myeloperoxidase immunoreactivity in adult acute lymphoblastic leukemia. Am J Clin Pathol. 116(1):25-33. 2001-07-01 [PMID: 11447748]
• Lanza F. J Mol Med;76:676-81. 1998-01-01 [PMID: 9766845]
• Weil SC, Rosner GL, Reid MS et al. Translocation and rearrangement of myeloperoxidase gene in acute promyelocytic leukemia. Science;240:790-2. 1988-01-01 [PMID: 2896388]
• Kimura S, Ikeda-Saito M. Human myeloperoxidase and thyroid peroxidase, two enzymes with separate and distinct physiological functions, are evolutionarily related members of the same gene family. Proteins;3:113-20. 1988-01-01 [PMID: 2840655]
• Pinkus GS, Pinkus JL. Myeloperoxidase: a specific marker for myeloid cells in paraffin sections. Mod Pathol;4:733-41. 1991-01-01 [PMID: 1724087]

Bioinformatics

• Gene Symbol: MPO